Response of Aquatic Bacterial Species Isolated from Contaminaed Water to Whole Body Extract of Lucilia sericata Larvae | ||
| Egyptian Journal of Aquatic Biology and Fisheries | ||
| Article 64, Volume 29, Issue 1, January and February 2025, Pages 1181-1199 PDF (458.33 K) | ||
| DOI: 10.21608/ejabf.2025.409622 | ||
| Author | ||
| Zidan et al. | ||
| Abstract | ||
| Drainage microbial contamination has been identified as a serious human health risk that have a detrimental impact on health issues brought on by the invasion of pathogenic organisms and are associated with wound infection. Ingestion or contact of polluted water are the two ways that these bacteria might infect humans. Maggot debridement is a commonly used treatment around the world to clean wounds. Based on these data, the aim of this work was to identify the microbial contamination, examining gene expression and possible antibacterial properties of metabolites from Extraction/Secretions (ES) of Lucilia sericata larvae. In this study, the antibacterial activity of the ES of L. sericata larvae was examined against Gram-positive Streptococcus pneumoniae, Staphylococcus aureus, and Gram-negative E. coli, Pseudomonas aeruginosa bacteria, both in their sterilized and multi-antibiotic-resistant forms. The agar well diffusion method was utilized to assess the maggots' ES in comparison with the strains. A 2-D PAGE protein analysis was performed. Inhibition zones were observed for S.aureus (18.3±2.1mm), Streptococcus pneumoniae (13.4±0.58mm) and E. coli (20.4±2.0mm); however, the extract was unable to produce an inhibiton zone for P.aeruginosa. Different proteins with varying molercular mass (<20-150kDa) and pI (3.3-7) were observed using 2-D PAGE. Following a series of antibiotic treatments, we assessed lucifensin and attacin gene expression changes in the bacteria. The antibacterial impact was investigated using antibiotic disk diffusion and optical absorption by analyzing the expression of the previously known genes. Using Fluorescence-activated cell sorting analysis, various extract dilutions showed varying killing rates for S. aureus, S. pneumoniae, E. coli, and P. aeruginosa, with killing rates of 76, 71, 89.2, and 49.1% for the lowest (1/64) dilution, respectively. | ||
| Keywords | ||
| Aquatic bacteria; Aquatic microbial; contaminants; SDS-PAGE; MDT; Lucilia sericata; Gene expression; Larval debridement; Extraction/secretions; Anti-microbial effects | ||
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