ANTIMICROBIAL ACTIVITY OF LECTIN ISOLATED FROM EGYPTIAN WHEAT CULTIVAR GIZA 171 | ||
| Zagazig Journal of Agricultural Research | ||
| Article 14, Volume 46, Issue 3, May and June 2019, Pages 783-792 PDF (1.23 M) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/zjar.2019.40968 | ||
| Authors | ||
| Safa A. Mostafa* ; H. A. Badr; A. Osman; K. M. Wahdan | ||
| Agric. Biochm. Dept., Fac. Agric., Zagazig Univ., Egypt | ||
| Abstract | ||
| In the current study crude lectin was isolated from Egyptian wheat seeds cultivar Giza 171 and it was characterized by several methods such as sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), Native-PAGE, Iso-electric point evaluation, Fourier transform infrared (FTIR) spectroscopy and amino acids composition. Furthermore, antibacterial and antifungal activities were estimated against some gram positive, gram negative bacteria and pathogenic fungus. Based on Native-PAGE (unreduced) of wheat seeds lectin indicates one major band with a molecular weight of approximately 50 kDa. SDS-PAGE of wheat seeds lectin separated this high molecular weight band into 2 bands corresponding to smaller molecular weights (14,20 kDa). The iso-electric points were deduced from the protein pH-solubility curve. It is clear that the least soluble points were obtained at pH 5.5. The content of the hydrophobic amino acids residues (proline, valine, isoleucine, leucine and phenyl alanine) valued 27.8% and this represent 31.1% of the total amino acids. The minimum inhibitory concentrations (MIC) of lectin against L. monocytogenes and P. aeruginosa was 800 μg/ml, 1600 μg/ml for B. subtillis and 3200 μg/ml against E. coli. Lectin inhibited mycelial growth of F. oxysporun and F. solani at a wide concentration range (250, 500 and 1000 μg/ml). This study has elucidated that the lectin isolated from wheat seeds cultivar Giza 171 have the potent antibacterial and antifungal activities against selected pathogenic bacteria and fungai. | ||
| Keywords | ||
| Wheat; Lectin; Antibacterial; Antifungal; SDS-PAGE | ||
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