E. coli clinical isolates: Phylogrouping and virulence factors in Alexandria, Egypt | ||
| Microbes and Infectious Diseases | ||
| Articles in Press, Accepted Manuscript, Available Online from 04 February 2025 | ||
| Document Type: Original Article | ||
| DOI: 10.21608/mid.2025.352346.2458 | ||
| Authors | ||
| Shahinda Rezk* 1, 2; Ahmed Jamal1; Abeer Ghazal1; Aliaa Gamaleldin Aboulela1 | ||
| 1Department of Microbiology, Medical Research Institute, Alexandria University, Egypt. | ||
| 2Institute of Virology, Medical Center-University of Freiburg, Freiburg 79104, Germany | ||
| Abstract | ||
| Background: E. coli is can be categorized into many pathotypes which represent groups of strains with specific pathogenic characteristics. This study aimed to identify phylogroups and different virulence factors in E coli isolated from clinical specimens. Methods: Fifty clinical E. coli isolates were included in this study. Identification and Antibiotic susceptibility testing were performed by Vitek® 2 compact system. Virulence genes detection and phylogroup gene alleles were performed using SYBR Green Real-time PCR. Results: The results revealed high antibiotic resistance; 62% of the isolates were MDR and 8% were XDR. The most prevalent virulence gene was papG (78%) while the least was hlyA (22%). Phylogroup B2 was the most predominant (52%), followed by (10%) group D, (8%) group C and group E each, (6%) group B1, (4%) clade1 and (2%) group F. Conclusion: Phylogroup B2 had all the virulence genes investigated in this study and it was more associated with sat and hlyA virulence genes. | ||
| Keywords | ||
| Phylogroups; Virulence genes; Antibiotic resistance | ||
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