Restriction Fragment Length Polymorphism of DRB3 Exon 2 of BoLA Class II Gene Complex in Relation to Phenotypic Traits of Holstein Dairy Cows | ||
| Egyptian Journal of Veterinary Sciences | ||
| Articles in Press, Corrected Proof, Available Online from 11 February 2025 PDF (949.15 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/ejvs.2024.294931.2144 | ||
| Author | ||
| Mahmoud Hashad* | ||
| Professor of Microbiology, Faculty of Veterinary Medicine, Cairo University | ||
| Abstract | ||
| In this study, PCR-RFLP was performed to detect the potential association among the genotypic polymorphism of BoLA-DRB3 exon 2 in 20 Holstein dairy cows and recurrent cystic ovary, mastitis, milk production and foot and mouth disease virus infection. Based on the combination between the RFLP patterns produced by two restriction enzymes, 5 patterns were seen. The profile AB showed the highest incidence of recurrent cystic ovary, as four animals needed mating for three times for conception. Concerning the milk yield, a cow with the yield (BB profile showed the highest 5200 Kg/305 days) followed by those with the BC profile. Cows with the RFLP profiles AA, AB and BB did not attract FMD infection during the endemics that attacked the flock. Meanwhile, two cows with the BC profiles showed severe FMD symptoms. Animals with the rest of profiles showed mild to moderate FMD symptoms according to the farm records. It is noticed that cows with profiles AA, AB and BB were affected with clinical mastitis 3 to 4 times through the production period (305 days). Cows with the profile BC were the least affected with subclinical mastitis. In conclusion, polymorphism of BoLA-DRB3 exon 2 in the studied Holstein cows showed sorts of association with productive and immunological traits and can be a reliable genetic marker for selection of animals with better traits. However, more investigation is needed on a bigger number of animals and more traits. | ||
| Keywords | ||
| BoLA DRB3; RFLP; PAGE; Holstein cows; phenotypic traits | ||
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