Abugrara, A., Hassan, H. (2024). Evaluation of the effect of using soaked grated beans (SGB) as a nitrogen source and sodium bicarbonate (SB) as a carbon source on the chemical composition, fatty acid, and amino acid content of the marine microalga Nannochloropsis oceanica.. test, 11(2), 50-57. doi: 10.21608/maj.2024.411270
Ali, M. Abugrara; Habashy, M. Hassan. "Evaluation of the effect of using soaked grated beans (SGB) as a nitrogen source and sodium bicarbonate (SB) as a carbon source on the chemical composition, fatty acid, and amino acid content of the marine microalga Nannochloropsis oceanica.". test, 11, 2, 2024, 50-57. doi: 10.21608/maj.2024.411270
Abugrara, A., Hassan, H. (2024). 'Evaluation of the effect of using soaked grated beans (SGB) as a nitrogen source and sodium bicarbonate (SB) as a carbon source on the chemical composition, fatty acid, and amino acid content of the marine microalga Nannochloropsis oceanica.', test, 11(2), pp. 50-57. doi: 10.21608/maj.2024.411270
Abugrara, A., Hassan, H. Evaluation of the effect of using soaked grated beans (SGB) as a nitrogen source and sodium bicarbonate (SB) as a carbon source on the chemical composition, fatty acid, and amino acid content of the marine microalga Nannochloropsis oceanica.. test, 2024; 11(2): 50-57. doi: 10.21608/maj.2024.411270

Evaluation of the effect of using soaked grated beans (SGB) as a nitrogen source and sodium bicarbonate (SB) as a carbon source on the chemical composition, fatty acid, and amino acid content of the marine microalga Nannochloropsis oceanica.

Mediterranean Aquaculture Journal
Volume 11, Issue 2, December 2024, Pages 50-57    PDF (352.64 K)
Document Type: Original Article
DOI: 10.21608/maj.2024.411270
Authors
Ali, M. Abugrara1; Habashy, M. Hassan* 2
1Department of Marine Resources, Faculty of Natural Resources and Environmental Sciences, Omar Al-Mokhtar University, El-Beda, Libya
2Department of Animal and Fish Production, Faculty of Agriculture, Alexandria University, Alexandria, Egypt
Abstract
Microalgae culture media should be cost-effective, enable high growth, meet precise requirements, and be readily available. In this study, the effect of different levels of sodium bicarbonate (SB) and Soaked grated beans (SGB) in the growth medium on the biochemical components (protein, carbohydrate, lipid, fatty acids, and amino acids) of Nannochloropsis oceanica was evaluated compared to standard Gaillard F/2 medium. Treatments were performed as follows: - control (OCO): Gaillard F/2 medium, OD1: 75 % of SB+25% of SGB, OD2: 50% SB+50% of SGB and OD3: 25%SB+75%of SGB. The results obtained revealed that the biochemical components of N. oceanica are affected by the level of sodium bicarbonate and bean sprout infusion. The highest protein and carbohydrate contents were obtained from the OD3 treatment, the highest lipid content from the OD1 treatment and the highest EAA content (54.41%) were obtained using OD3 medium compared to the control group (100% F/2). The highest biomass productivity was obtained from OD3 treatment followed by OD2 and the highest total saturated fatty acids (TSFA) content of N. oceanica was recorded by OD2 medium.
 
Keywords
Keywords: Amino acids; Fatty acids; N. oceanica; Proximate composition
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