Isolation, identification, and a statistical optimization of scleroglucan-producing Athelia rolfsii Did-2 strain isolated from infected Phaseolus vulgaris. | ||
| Egyptian Journal of Chemistry | ||
| Articles in Press, Accepted Manuscript, Available Online from 16 February 2025 | ||
| Document Type: Original Article | ||
| DOI: 10.21608/ejchem.2025.330719.10685 | ||
| Authors | ||
| ahmed Eldidamony1; mohamed kalaba1; islam a elsehemy* 2; Saad Moghannem3; Mohamed A. M. Farid4 | ||
| 1Botany and Microbiology Department, Faculty of Science, Al-Azhar University, Cairo 11884, Egypt. | ||
| 2National Research Centre, Chemistry of Natural and Microbial Products Department, Dokki 12622, Cairo, Egypt | ||
| 3Botany and Microbiology Department, Faculty of Science, Al-Azhar University, Cairo - 11884, Egypt | ||
| 4Natural and microbial products department, National research center | ||
| Abstract | ||
| Athelia rolfsii Did-2 (OP600167) is a fungal strain that produces Scleroglucan, a non-ionic water-soluble biopolymer used in various industries. The study aimed to isolate and identify this fungal strain. Five fungal isolates from infected plants were tested, and strain Did-2 was selected as the highest producer (1.43 g/100 mL). The strain was identified using various international keys, including macroscopic, microscopic, and molecular studies. The partial sequence was deposited in GeneBank under the name Athelia rolfsii strain Did-2 18S ribosomal RNA gene with accession number OP600167. To maximize Scleroglucan yield, the Plackett-Burman design (PBD) and the central composite design response surface method (RSM) were employed. The optimized medium resulted in a 2-fold improvement (2.651 g/100 mL) in Scleroglucan yield | ||
| Keywords | ||
| Athelia rolfsii; scleroglucan; optimization Plackett-Burman; Response surface methodology | ||
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