Evaluation of the antimicrobial activity of Lactobacillus strains against multidrug resistant Klebsiella and Pseudomonas species | ||
| Microbes and Infectious Diseases | ||
| Articles in Press, Accepted Manuscript, Available Online from 25 February 2025 | ||
| Document Type: Original Article | ||
| DOI: 10.21608/mid.2025.351516.2452 | ||
| Authors | ||
| Mona Gamal Nada; Maha Mahmoud Kotb* | ||
| Department of Medical Microbiology and Immunology, Faculty of Medicine, Cairo University, Egypt | ||
| Abstract | ||
| Background: The rise of multidrug-resistant (MDR) pathogens has encouraged more research to identify harmless and a potential substitute to antibiotics, such as probiotics. The current study aims to evaluate the inhibitory activity of cell-free supernatants (CFS) of Lactobacillus acidophilus (L. acidophilus) and Lactobacillus fermentum (L. fermentum) against MDR Klebsiella species and Pseudomonas species. Methods: The current study included a total of 52 (26 each) clinical isolates of Klebsiella and Pseudomonas, confirmed to be MDR by Kirby-Bauer disc diffusion method. Anti-bacterial activities of the CFS of L. acidophilus (DSM 20079) and L. fermentum (ATCC 9000338) against the MDR isolates were assessed by agar well diffusion. Detection of biofilm formation by test pathogens was done by the tissue culture plate (TCP) method and the CFS ability to interfere with biofilm production was studied. Results: The agar well diffusion test showed that the mean value of inhibition zone diameter of L. fermentum and L. acidophilus against Klebsiella were 14.3 mm and 13.82 mm respectively. While the mean value of inhibition zones of L. fermentum and L. acidophilus against Pseudomonas were 14.29 mm and 15.45 mm respectively. Biofilm production was identified in 73.1% of the isolates. L. fermentum and L. acidophilus revealed reductions of 46% and 47.2%, respectively, in biofilm production in Klebsiella. While L. acidophilus was more effective (61%) in inhibiting the formation of biofilm in Pseudomonas than L. fermentum (46.5%). Conclusions: L. fermentum and L. acidophilus have an important anti-bacterial effect against MDR Klebsiella species and Pseudomonas species. | ||
| Keywords | ||
| Cell-free supernatants; probiotics; inhibitory activity; Gram negative bacilli; biofilm | ||
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