Development of a Novel Oil Formulated from Thirteen Chemical Compounds: Economic Significance of Active Compound Quantities Based on Synergy Studies | ||
| Bulletin of Pharmaceutical Sciences Assiut University | ||
| Articles in Press, Accepted Manuscript, Available Online from 08 March 2025 | ||
| Document Type: Original Article | ||
| DOI: 10.21608/bfsa.2025.338491.2352 | ||
| Authors | ||
| Ahmed Aityahia1; Abdallah Abdallah el hadj* 1; Hamza Kahina1; Salah Hanini2; Alma MEHMET HAKKI3; Ramazan ERENLER3 | ||
| 1Department of chemistry, Faculty of Science, University of Blida-1 | ||
| 2Laboratory of Biomaterials and Transport Phenomena, Yahia Fares University of Medea, Algeria. | ||
| 3Researcher Laboratory Application and Researcher Center, Faculty of Health Sciences, Iğdir University, Iğdir, Turkey | ||
| Abstract | ||
| In this work, oil formulated from thirteen standard compounds is developed. These compounds include seven monoterpene hydrocarbons (limonene, α-pinene, β-pinene, p-cymene, myrcene, camphene, ocimene), two monoterpenic alcohols (menthol, linalool), two monoterpenic phenols (carvacrol, thymol), one monoterpene ketone (camphor), and one phenylpropene (eugenyl acetate). The evaluation of the antioxidant activity of these pure compounds, using the free radical trapping method 2,2-diphenyl-1-picrylhydrazyl (DPPH), shows that phenolic compounds are the most active; however, their activities remain inferior to those of reference antioxidants (BHT, BHA, and guaiacol). The activity of the formulated oil demonstrates a significant positive synergistic effect, closely matching the activity of guaiacol. This synergistic effect results in a marked decrease in the EC50 of the formulated oil to a value of 27 μmol/ml, with the interactions resulting from the difference in antioxidant activity between experimental and theoretical values (percentage difference in DPPH) equal to 94.62%. This corresponds to a decrease in the overall active dose of 99.95%. Although the partial synergistic contributions of phenolic compounds are the most significant—carvacrol (79.69%), thymol (64.87%), and eugenyl acetate (19.81%)—those of other phenolic compounds are generally less than or equal to 1%. Nonetheless, these compounds induce a material gain in the quantity of compounds with the highest reducing potential, yielding decreases (that is, gains in active materials) of 20.31%, 35.13%, and 80.19%, respectively. | ||
| Keywords | ||
| Formulated Oil; DPPH; Antioxidant activity; Reducing potential; synergistic contributions | ||
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