Loop-mediated isothermal amplification for protozoan diagnosis: review of ongoing development and implementations | ||
| Parasitologists United Journal | ||
| Articles in Press, Accepted Manuscript, Available Online from 12 March 2025 | ||
| Document Type: Review Article | ||
| DOI: 10.21608/puj.2025.338230.1278 | ||
| Authors | ||
| Mona K Hegazy* 1; Ahmed Gomaa2; Nora E Saleh3 | ||
| 1Medical Parasitology Department, at Mansoura faculty of Medicine, Egypt. | ||
| 2Department of Medical Parasitology, Faculty of Medicine, Mansoura University, Mansoura, 35516, Egypt | ||
| 3Department of Medical Parasitology, Faculty of Medicine, Mansoura University | ||
| Abstract | ||
| Parasitic protozoa are emerging as global health risks worldwide. Accurate screening of protozoal infection is pivotal for an effective strategic control process. Traditional parasitological techniques, immunodiagnostics and PCR-based methods are the classical tools for diagnosing protozoal infection, even so they are labor and time-intensive, expensive, and occasionally give inconsistent results. The loop mediated isothermal amplification (LAMP) assay, is a single-step amplification reaction that is an extremely sensitive, efficient and robust technique for identification of protozoa. This review aims to offer an overview of the present status of LAMP technology on protozoan diagnosis and to highlight this innovative tool and its future progress through comparison with other traditional diagnostic techniques. Moreover, LAMP essential principles were described and its fundamental goals, i.e. sensitivity, specificity, and overall diagnostic accuracy in comparison with conventional diagnostic techniques for protozoan infections were discussed. | ||
| Keywords | ||
| Amplification; DNA; isothermal conditions; LAMP; molecular diagnostics; PCR; primers; protozoa | ||
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