Sero- evaluation of cattle cystic echinococcosis using different antigens of hydatid cyst fluid, protoscolices, germinal and laminated layers | ||
| Egyptian Veterinary Medical Society of Parasitology Journal (EVMSPJ) | ||
| Volume 21, Issue 1, 2025, Pages 1-15 PDF (744.13 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/evmspj.2025.417342 | ||
| Abstract | ||
| Cystic echinococcosis (CE), caused by Echinococcus granulosus, affects both humans and livestock. Final hosts (dogs) are typically subclinical, while intermediate hosts show symptoms based on cyst location and infection severity. CE is mainly detected in abattoirs via post mortem (PM) examination, making diagnosis challenging and increasing transmission risk. The current study aimed to evaluate the serodiagnosis and statistical significance of using different prepared antigens from cysts against PM method. 170 blood samples were collected from cattle and the separated serum samples were prepared for detection of optical density values by indirect ELISA against different hydatid cysts antigens. The antigens were isolated from various cystic components. Crude hydatid cyst fluid antigen (HCF Ag) was prepared by aspirating fluid from fertile cyst separated from infected organs, centrifuged and aliquoted, laminated layer antigen (LL Ag) obtained by aspirating fluid, separating the laminated layer and homogenized, germinal layer antigen (GL Ag) was isolated through dissection, protoscolices antigen (Pscs Ag) collected, washed with PBS, and all isolates sonicated, and stored at -20°C. Protein concentration was measured using modified Lowry method. The obtained values were statically analyzed. PM prevalence was 11.76%. While ELISA showed 13.5%, with higher prevalence in the liver (7.06%) than lungs (2.35%). Significant differences were noted between antigens, with GL, HCF, and Pscs Ags showing higher values than LL Ag. ELISA diagnostic accuracy was 98.24 % for LL, GL, and Pscs Ags, and 60% for HCF Ag. Our study suggests that GL, HCF, and Pscs Ags were effective and sensitive immunodiagnostic tools for screening hydatosis. | ||
| Keywords | ||
| Cystic Echinococcosis; Cysts; Echinococcus Infection; Hydatid; Hydatidosis | ||
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