Determination of minimum inhibition concentration of antimicrobial for detecting resistance in Escherichia coli isolated from women with urinary tract infections | ||
| Microbes and Infectious Diseases | ||
| Articles in Press, Accepted Manuscript, Available Online from 19 March 2025 | ||
| Document Type: Original Article | ||
| DOI: 10.21608/mid.2025.353118.2463 | ||
| Authors | ||
| Jilan Ahmed Shakur1; Zainab Kareem Ahmed2; Wael Adil Obaid* 3 | ||
| 1College of Medical Laboratory Techniques. Imam Jaafar AI-Sadiq University, Kirkuk, Iraq | ||
| 2Department of Biology, College of Education-Tuz Hurmatu, Tikrit University, Iraq | ||
| 3Department of Applied Pathological Analysis, College of Science, Al-Nahrain University, Jadiriya, Baghdad, Iraq | ||
| Abstract | ||
| Background: The bacteria is importance causes to urinary tract infections (UTIs), in women and older adults, leading to different a significant to the financial expenses incurred by patients or healthcare systems. Uropathogenic Escherichia coli (UPECs) is the common bacteria that causes urinary tract infections (UTIs). that return for different main causative agent, initially this bacterium colonizes and adhering to the bladder epithelium of human host. Aim: Determination of MIC to different type of Antimicrobial to detect Escherichia coli resistance. Methods: Twenty samples of urine were placed in sterile urine cups cultured on Macconkay agar, Eosin methylene blue and blood agar then confirmed E. coli identification through DL microbial ID/AST system (Enterobact. IST CARD) model DL-120E and detected MIC of E. coli. Result: Diagnosed (15) isolates of Escherichia coli taken it from urine clinical sample from Woman, are resistance to some antibiotic that include (Cefazolin, Nitrofurantoin, Ampicillin, Gentamicin, Ampicillin-Sulbactam, Piperacillin-Tazobactam, Cefepime, Cefoxitin, Cefuroxime, Cefuroxime, Levofloxacin, Trimethoprim-Sulfamethoxazole, Cefotaxime, Imipenem, Meropenem, Amikacin, Ertapenem, Ceftazidime, Cefoperazone-Sulbactam, Minocycline, Polymixin B, Chloramphenicol, Ceftazidime-Clavulanic acid, Cefotaxime-Clavulanic acid, Tigecycline and Azithromycin) and found (15) isolates of E. coli with percentage 100% sensitivity to Nitrofurantoin, Ampicillin, Gentamicin, Piperacillin-Tazobactam, Trimethoprim-Sulfamethoxazole, Ceftazidime and Minocycline and all isolates with percentage 100% also resistance to Cefazolin, Cefepime, Cefuroxime(vi), Cefuroxime( oral), Levofloxacin, Cefotaxime, Imipenem, Meropenem, Amikacin, Ertapenem and Azithromycin. Conclusion: The current study has shed light on a crucial finding, E. coli isolates out as the most significant bacterium isolated from urine samples of women infected with UTIs. This bacterium's resistance to different type of antimicrobial in light of this, further must induction research into E. coli is essential, focusing on understanding its resistance genes and plasmids to curb the potential spread of this disturbing bacterium. | ||
| Keywords | ||
| E. coli; MIC; antibiotic resistance; susceptibility | ||
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