Detection of blaNDM-1 gene in Escherichia coli and Klebsiella pneumoniae Producing Extended Spectrum β-lactamase (ESBL) Isolated from Urine Samples | ||
| Egyptian Journal of Medical Microbiology | ||
| Volume 34, Issue 3, July 2025 | ||
| Document Type: New and original researches in the field of Microbiology. | ||
| DOI: 10.21608/ejmm.2025.367538.1516 | ||
| Authors | ||
| Aya T. Mahmoud* 1; Azza A. Gomaa1; Rabie M.A. Ibrahim2; Mostafa S. Sheemy1 | ||
| 1Medical Microbiology and Immunology Department, Faculty of Medicine, Beni-Suef University, Egypt | ||
| 2Urology Department, Faculty of Medicine, Beni-Suef University, Egypt | ||
| Abstract | ||
| Background: The most bacterial species showing resistance to almost all commonly used antibiotics are members of the Enterobacteriaceae family, including Klebsiella pneumoniae and Escherichia coli. One of the few treatment of emerging MDR bacteria is carbapenem. Objective: We aimed to find out the occurrence the carbapenem-resistant blaNDM-1 gene in ESBL-producing E. coli and K. pneumoniae isolated from urinary tract infections. Methodology: We tested four hundreds and sixty patients with UTIs at the Urology department of Beni-Suef university Hospital. The urine samples are subjected for bacteriological culture and identification of isolated oganisms. Isolates were tested for ESBL and MBL (Metallo-β lactamase) producing Escherichia coli and Klebsiella pneumoniae. We used polymerase chain reaction (PCR) to check whether the bla-NDM-1 gene was present in the MBL generating colonies. Results: E. coli was detected in 155 (33.7%) urine samples and K. pneumoniae in 92 (20%). E. coli had a prevalence rate of 38.1% for ESBL and K. pneumoniae had a rate of 39.1%. Additionally, 53.33 percent of the E. coli and 76.7 percent of the K. pneumoniae showed phenotypic positivity for MBL production. The percentage of E. coli and K. pneumoniae that tested positive for BlaNDM-1 was 71.8 and 75.0 percent, respectively, according to PCR results. Conclusion: Findings indicate that the blaNDM-1 gene is more common than previously thought. Hospitals and communities must immediately implement screening tests to determine the prevalence of blaNDM-1 dissemination. | ||
| Keywords | ||
| Bla-NDM-1; ESBL; E.coli; K. pneumoniae | ||
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