A Histological Study of the Impact of Bone Marrow Mesenchymal Stem Cells- Derived Exosomes on the Corpus Callosum in a Cuprizone-Induced Mouse Model of Multiple Sclerosis | ||
| Egyptian Journal of Histology | ||
| Articles in Press, Accepted Manuscript, Available Online from 23 March 2025 | ||
| Document Type: Original Article | ||
| DOI: 10.21608/ejh.2025.366901.2225 | ||
| Authors | ||
| Bosy Ahmed Abdelaziz Hassan* 1; Nagwa Mohamed El Shakaa2; Gehad Ahmed Hammouda3; Mona Hussien Raafat Ahmed4 | ||
| 1Histology department, Faculty of Medicine, Ain shams university, Cairo, Egypt. | ||
| 2Histology department, faculty of Medicine, Ain Shams university , Cairo, Egypt | ||
| 3Histology Department, Faculty of Medicine, Ain Shams University. Cairo, Egypt | ||
| 4Histology Department, Faculty of Medicine, Ain Shams university, Cairo, Egypt | ||
| Abstract | ||
| Introduction: A chronic demyelinating disease that affects millions of peoples worldwide is multiple sclerosis (MS). There are currently no remyelination therapy for this disease, and exosomes are being explored as an alternative. Aim: to study how exosomes generated from bone marrow mesenchymal stem cells (BMSCs) affect the corpus callosum structure in male mice with cuprizone model of MS. Materials and methods: Forty-seven adult male mice were used in this work. Exosomes were isolated and extracted from five mice. Forty-two adult male mice were split into two equal groups randomly: Group I (Control): divided into three equal subgroups. Group II (Cuprizone-treated): which was treated with cuprizone, was given 400 mg/kg/day of the drug orally every day for 8 weeks. Then equally divided into three subgroups. Subgroup IIA: sacrificed 24 hours following the final cuprizone dosage. Subgroup IIB (Recovery): left without treatment for further 4 weeks, then were sacrificed. Following the final dose of cuprizone, subgroup IIC (exosomes treated): got a single intravenous injection of exosomes into tail vein, and were sacrificed 4 weeks later. After scarification, brains tissue were prepared using histological, immune-histochemical methods and histomorphometric studies. Results: Histological analysis of corpus callosum in subgroups IIA & IIB showed damaged nerve fibers by H&E with multiple vacuoles. Improvement of these changes was noticed in subgroup IIC. By luxol fast blue, nerve fibers stained deep blue in subgroup IIC & control group. While, in subgroups IIA & IIB, nerve fibers stained faint blue. Subgroups IIA & IIB showed marked rise in the number of GFAP and Iba1 positive cells. Conclusions: In the cuprizone model of MS, exosomes were demonstrated to improve demyelination of mice's corpus callosum. Exosomes may therefore offer a novel approach to MS treatment. | ||
| Keywords | ||
| MS; Cuprizone; Corpus callosum; BMSCs derived exosomes; GFAP | ||
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