An Easy and Simple Approach for Biosynthesis of Selenium Nanoparticles; Characterization, Radiolabeling and Biomedical Application | ||
| Egyptian Journal of Chemistry | ||
| Articles in Press, Accepted Manuscript, Available Online from 25 March 2025 | ||
| Document Type: Original Article | ||
| DOI: 10.21608/ejchem.2025.338679.10892 | ||
| Authors | ||
| Heba Ibrahim Elkhouly* 1; zeinab s. Nasr2; Mohamed K. Hassane3 | ||
| 1Yossuf Abbas St. | ||
| 2Labeled Compounds Department., Hot Labs Center, Egyptian Atomic Energy Authority (EAEA), Cairo, Egypt. | ||
| 3Radioisotopes Production Facility (RPF), Egyptian Second Research Reactor (ETRR‑2), Atomic Energy Authority, P.O. Box 13759, Cairo, Egypt | ||
| Abstract | ||
| Nanomaterials biogenic approaches of synthesis exceed chemical ways in simplicity, toxicity and generation of homogeneous colloidals. Herein, a rare approach for biosynthesis of SeNPs is described where a rapid myogenic method was applied. The endophytic fungus isolate, Asperigillus terrus H1 with accession no. PQ550684, that isolated for the first time from Rumex thyrsiflorus was used for the bio-reduction of sodium selenite contained in the growth medium to produce selenium nanoparticles. Change in color of the culture medium to orange-red signifies the formation of nano-selenium. DLS, TEM and ICP-OES techniques demonstrated the formation of monodispersive amorphous selenium nanoparticles with average hydrodynamic size of 55.76 nm with -25.6 particles charge. The synthesized SeNPs were checked for their in-vitro cytotoxicity against normal human lung fibroblast cells (MRC-5) and human lung adenocarcinoma cell line (A549) which giving IC50 of 485.16±13.71 and 196.25± 4.23 µg/ml respectively. Their ability to be applied in biomedical applications was studied in-vivo through labeling them with 99mTc and tracing their distribution in normal and tumor-bearing mice. The produced mycogenic SeNPs showed excellent physical and biological characteristics that could make them a promising nanoplatform for tumor molecular imaging. | ||
| Keywords | ||
| Biogenic nanoparticles; Selenium; endophyte Asperigillus terrus H1; Tumor Imaging; Radiolabeling | ||
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