Efficacy of flaxseed oil alone or loaded on ZIF-8 nanoparticles on experimental cryptosporidiosis | ||
| Parasitologists United Journal | ||
| Articles in Press, Accepted Manuscript, Available Online from 26 March 2025 | ||
| Document Type: Original Article | ||
| DOI: 10.21608/puj.2025.361671.1286 | ||
| Authors | ||
| Fayza El-Sayed1; Amira Elghannam2; Ghada Omar2; reda abdelhameed3; ashraf barakat4; sabry sadek5; Amira Thanaa Ali* 6 | ||
| 1Department of Medical Parasitology, Faculty of Medicine, Benha University, Benha, Qalubyia, Egypt | ||
| 2Department of Parasitology, Faculty of Medicine, Benha University, Benha, Qalubyia, Egypt | ||
| 3national research center | ||
| 4Department of Zoonotic Diseases, National Research Centre, Dokki, Giza, Egypt | ||
| 5Zoonotic Diseases Department, National Research Centre, Dokki, Giza, Egypt | ||
| 6Parasitology department, faculty of medicine, Benha University | ||
| Abstract | ||
| Background: Cryptosporidium is one of the leading causes of waterborne diarrheal disease outbreaks globally. Although the parasite is highly prevalent, the only approved drug is nitazoxanide (NTZ), which is not effective in treating cryptosporidiosis in immunocompromised individuals with moderate effectiveness in immunocompetent individuals. Objective: To evaluate the efficacy of flaxseed oil alone or loaded on nanoparticles in Cryptosporidium-infected mice. Materials and Methods: This study was conducted on 60 immunocompromised mice divided into 6 groups (10 mice each): Group 1: Non infected non treated; Group 2: Infected non treated; Group 3: Infected and given NTZ (100 mg/kg/day); Group 4: Infected and given flaxseed oil (300 mg/kg/day); Group 5: Infected and given combination of NTZ and flaxseed oil; Group 6: Infected and given flaxseed oil loaded on ZIF-8 nanoparticles. Oocyst shedding, antioxidant enzyme SOD, oxidative stress marker MDA, and intestinal histopathology were investigated. Results: Flaxseed oil decreased oocyst count in mice stool, increased SOD and decreased MDA in mice serum. It also improved intestinal inflammatory changes as a result of cryptosporidiosis. Conclusion: Adding flaxseed oil to NTZ improves the outcome of cryptosporidiosis. Moreover, ZIF-8 has proven to be a successful drug delivery method. | ||
| Keywords | ||
| Cryptosporidium; flaxseed oil; ZIF-8 nanoparticles; NTZ; oxidative stress markers | ||
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