Molecular comparison of some virulence factor genes of gram-negative bacterial species causing urinary tract infections from patients with type 1 and type 2 diabetes | ||
| Journal of Bioscience and Applied Research | ||
| Articles in Press, Accepted Manuscript, Available Online from 25 March 2025 | ||
| Document Type: Original Article | ||
| DOI: 10.21608/jbaar.2025.420137 | ||
| Authors | ||
| Musaab Obaid Hamad* 1; Mohammed Nadhir Maaroof2 | ||
| 1Directorate General of Education in Nineveh /Mosul High school for Superiors Students, Mosul, Iraq | ||
| 2Department of Biology, College of Sciences /Tikrit University, Tikrit, Iraq | ||
| Abstract | ||
| Background: The incidence of urinary tract infections is high in Diabetic Patients. Bacterial pathogens that cause UTIs have several virulence factors; knowledge of these virulence factors at a molecular level is important to enhance the elimination of bacterial infection. Materials and methods: One hundred fifty urine samples were collected, 58 of them from type 1 diabetic patients and 92 from type 2, Bacterial isolates were diagnosed. Molecular detection of virulence factors was done. Results: The highest causative agent of UTIs was E. coli; β-lactamase was highest (86%) in the causative agent of UTIs in type 1 diabetic patients, while biofilm formation was highest (82.8%) in the causative agent in type 2. The genes rsmA and ureR appeared in 100% of Proteus mirabilis isolated from both type of diabetic patients separately, The gene ureE appeared in 100% of Morganella morganii isolated from both types of diabetic patients separately, The ureD gene was appeared in 100% and 80% in Klebsiella pneumoniae isolated from type 1 and 2 diabetic patients respectively, as for fimH gene it was 100% and 70% of Klebsiella pneumoniae isolated from type 1 and 2 diabetic patients respectively. The ndvB gene has appeared in 60% and 42.8% of Escherichia coli isolated from type 1 and 2 diabetic patients, respectively. Conclusion: The ndvB, blaTEM, fimH and uerD genes observed in different proportions between isolates isolated from both types of diabetic patients. We found ureR, rsmA, and ureE genes in a similar ratio. | ||
| Keywords | ||
| UTIs; Diabetic Patients; Molecular variation; PCR | ||
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