El-Garawani, I., El-Sabbagh, S., Abbas, N., Zayed, M., Elsayed, A., Ahmed, H. (2020). Biochemical characterization and anticancer activity of newly isolated haloalkaliphilic bacterial strain against hepatocellular carcinoma in vitro. test, 2(3), 39-47. doi: 10.21608/jmals.2020.420337
Islam M. El-Garawani; Sabha M. El-Sabbagh; Nasser H. Abbas; Muhammad Zayed; Afaf Elsayed; Hany S. Ahmed. "Biochemical characterization and anticancer activity of newly isolated haloalkaliphilic bacterial strain against hepatocellular carcinoma in vitro". test, 2, 3, 2020, 39-47. doi: 10.21608/jmals.2020.420337
El-Garawani, I., El-Sabbagh, S., Abbas, N., Zayed, M., Elsayed, A., Ahmed, H. (2020). 'Biochemical characterization and anticancer activity of newly isolated haloalkaliphilic bacterial strain against hepatocellular carcinoma in vitro', test, 2(3), pp. 39-47. doi: 10.21608/jmals.2020.420337
El-Garawani, I., El-Sabbagh, S., Abbas, N., Zayed, M., Elsayed, A., Ahmed, H. Biochemical characterization and anticancer activity of newly isolated haloalkaliphilic bacterial strain against hepatocellular carcinoma in vitro. test, 2020; 2(3): 39-47. doi: 10.21608/jmals.2020.420337

Biochemical characterization and anticancer activity of newly isolated haloalkaliphilic bacterial strain against hepatocellular carcinoma in vitro

Journal of Medical and Life Science
Volume 2, Issue 3, September 2020, Pages 39-47    PDF (464.28 K)
Document Type: Original Article
DOI: 10.21608/jmals.2020.420337
Authors
Islam M. El-Garawani* 1; Sabha M. El-Sabbagh2; Nasser H. Abbas3; Muhammad Zayed2; Afaf Elsayed2; Hany S. Ahmed2
1Department of Zoology, Faculty of Science, Menoufia University, Menoufia 32511, Egypt
2Department of Botany and Microbiology, Faculty of Science, Menoufia University, Menoufia 32511, Egypt
3Department of Molecular Biology, Genetic Engineering and Biotechnology Research Institute, University of Sadat City, Sadat City 32958, Egypt
Abstract
Incidence and mortality rates of liver cancer vary widely geographically, making it the sixth most common cancer in the world. Microorganisms that grow in high-salinity environments can produce bioactive compounds for therapeutic applications in the pharmaceutical field. In this study, a bacterial strain, Marinobacter HA2, was isolated from El-Hamra Lake in Wadi El-Natrun, Egypt. The biochemical features and anticancer activity were investigated. The cytotoxicity of the crude extract was assessed using the HepG2 cell line and normal human peripheral blood lymphocytes (HPBL) in vitro. The IC50 of the crude extract using the MTT test on the HepG2 cell line was calculated at 82±1.4 µg/ml.
Keywords
Liver cancer; haloalkaliphilic bacteria; human peripheral blood lymphocytes; Marinobacter HA2; Wadi El-Natrun; HepG2
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