The positive impact of the fig leaves aqueous extract on the optic neuropathy in the aged rats. A biochemical, histological and immunohistochemical study | ||
| Egyptian Journal of Histology | ||
| Articles in Press, Accepted Manuscript, Available Online from 06 April 2025 | ||
| Document Type: Original Article | ||
| DOI: 10.21608/ejh.2025.359712.2211 | ||
| Authors | ||
| Manar Faried1; Ghada Adel Hegazy2; Nesrin Salman* 3 | ||
| 1Department of Anatomy and Embryology, Faculty of Medicine, Menoufia University, Menoufia, Egypt | ||
| 2Department of clinical Pharmacology, Faculty of Medicine, Menoufia University, Egypt | ||
| 3Department of Anatomy and Embryology, Faculty of Medicine, Menoufia University, Egypt | ||
| Abstract | ||
| Abstract Background: Aging is associated with development of most neurodegenerative disorders including optic neuropathy. Fig leaves extract (FLE) can attenuate the apoptotic and the inflammatory effects of aging induced optic neurodegeneration due to its antioxidant, anti-inflammatory and antiapoptotic properties. Objectives: Our study reveals the ameliorative effect of FLE on the aging induced optic neuropathy via biochemical, histological and immunohistochemical assessment. Methods: 40 adult male albino rats, 20 young rats 3 months age (180-200 grams) and 20 aged rats 24 months age (250 and 300 grams), divided into; Group I (n = 10 young rats): kept with no treatment. Group II (n = 10 young rats), received FLE at a dose 400mg/kg, once daily via gastric tube. Group III (n = 10 aged rats): kept with no treatment. Group IV (n = 10 aged rats): received FLE at a dose 400mg/kg, once daily via gastric tube. After 6 weeks, duration of the experiment, samples of blood were gathered for biochemical evaluation, then the optic nerves were dissected for histopathological and immunohistochemical assessments. Results: FLE administration improved all the examined parameters exhibited in the aged group. There was reduction in the elevated levels of MDA and IL-8 in aged group treated with FLE as compared with aged non treated group. In addition, histologically, optic nerve sections showed almost normal cells, nerve fibers with normal arrangement of myelin sheath and less vacuolated neuropil. Furthermore, aged group treated with FLE showed increase in toluidine blue intensity and down regulation in of GFAP, TNF- α and caspase 3 positive immunoreaction as compared with aged non treated group. Conclusion: FLE is a novel agent that promotes optic nerve myelination and regeneration Keywords: aging, optic neuropathy, FLE. | ||
| Keywords | ||
| aging; optic neuropathy; FLE | ||
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