THE CHANGES IN EPIDIDYMAL CAMEL SPERMATOZOA AS AFFECTED BY GREEN LASER IRRADIATION | ||
| Egyptian Journal of Animal Production | ||
| Article 7, Volume 62, Issue 1, January 2025, Pages 61-67 PDF (365 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/ejap.2025.365609.1108 | ||
| Author | ||
| Ahmed Seioudy* | ||
| Animal Production Research Institute | ||
| Abstract | ||
| Twenty Sudan camels (Camelus dromedarius) with forty testes were applied in this study. The aim of this research was to determine the impact of short-wave length green laser irradiation at various times from 0 to 10 minutes on epididymal spermatozoa quality in camel. After exposure of laser, motile, viable, acrosomal damage of spermatozoa (%), AST, ALT and ALP enzymes activity were determined under the incubation at 37°C for 8hours. The penetrating ability of spermatozoa that exposed to laser irradiation for various times into she-camel cervical mucus at 37°C for 4 hours was also checked. The achieved results displayed that exposure to 6 min of laser achieved the highest percentage of motile spermatozoa. While, exposure to 2 min achieved the lowest percentages of dead spermatozoa, acrosomal damage, AST, ALT and ALP enzymes. The advancement of incubation time reduced (P<0.05) motile spermatozoa (%). Meanwhile, increased dead spermatozoa (%), acrosomal damage (%), AST, ALT and ALP enzymes. The penetrating ability of spermatozoa into cervical mucus was better (P<0.05) at all groups than the control group during incubation. In conclusion, laser irradiation may be considered as a cheap and effective technique for improving the quality of camel semen by increasing the percentages of motile spermatozoa, livability, acrosomal integrity which can be used as an indicator to enhance the function of mitochondriato extend the survival of spermatozoa. | ||
| Keywords | ||
| Camel; epididymal spermatozoa; greenlaser irradiation; incubation | ||
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