The Implications of Nanomedicine in Cancer Therapy: Cytotoxic and Apoptotic Effects of Selenium Nanoparticles on Human Leukemia Cell Lines | ||
| Egyptian Journal of Chemistry | ||
| Articles in Press, Accepted Manuscript, Available Online from 23 April 2025 | ||
| Document Type: Original Article | ||
| DOI: 10.21608/ejchem.2025.367786.11443 | ||
| Authors | ||
| Mahmoud Moawad1; Afaf Altrawy* 2; Sameh H. Ismail3; Soha Abuelela4; Wael l S. Abd El Aa5; Abdel Rahman A. Abdel Rahman5; Ghada M. Nasr6 | ||
| 1National Cancer Institute, Department of Pathology, Cairo University, Egypt | ||
| 2Medical biotechnology, college of biotechnology , MUST | ||
| 3Faculty of Postgraduate Studies for nanotechnology, Cairo university. | ||
| 4Clinical pathology department, faculty of medicine ASU | ||
| 5Department of Molecular Diagnostics, Genetic Engineering, and Biotechnology Research Institute, University of Sadat City, Egypt. | ||
| 6Genetic Engineering, and Biotechnology Research Institute, Department of Molecular Diagnostics, University of Sadat City, Egypt. | ||
| Abstract | ||
| Background and objective: Selenium nanoparticles (SeNPs) have been observed to exhibit a pleiotropic action, whereby they induce apoptosis in tumor cells while simultaneously protecting healthy tissue cells against stress-induced cell death. The cells underwent a 24-hour treatment with SeNPs. This work objective was to the cytotoxic impacts of a specific selenium nanoparticle examination as a possible nanomedicine anticancer agent in inducing apoptosis in human leukemia cell lines. Methodology: The MTT test was utilized to conduct the assessment of cytotoxicity and cell viability. To gain insight into the molecular processes of apoptotic genes, namely Bcl-2, Bax, and Caspase3, their levels of expression were assessed using real-time reverse transcription polymerase chain reaction (RT-PCR). Results: The IC50 values of selenium nanoparticles on HL-60, K-562, and HS-5 cell lines were determined to be 3.01 ± 0.29, 2.59 ± 0.33, and 439.4 ± 15.8 μg/mL, respectively. The assessment of gene expression levels demonstrated a significant reduction in the anti-apoptotic Bcl-2 gene, accompanied by an elevation in the pro-apoptotic Bax and caspase3 mRNA gene expressions. Conclusion: This study presented preliminary findings about the notable antitumor efficacy of Selenium Nanoparticles against human leukemia cell lines. | ||
| Keywords | ||
| SeNPs, Bax , BCL-2, caspase3 , HL-60; K-562 , HS-5 | ||
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