Phenolic compounds, antioxidant, and antimicrobial activities of methanolic extract from the Saharan endemic plant Haloxylon scoparium Pomel | ||
| Bulletin of Pharmaceutical Sciences Assiut University | ||
| Articles in Press, Accepted Manuscript, Available Online from 25 April 2025 | ||
| Document Type: Original Article | ||
| DOI: 10.21608/bfsa.2025.355360.2418 | ||
| Authors | ||
| Kelthoum Ahmed-Gaid* 1; Nour El Yakine Ahmed-Gaid2; Saida Hanoun3; Houssem Chenna4; Khaoula Ghenaiet5; Hadjer Ahmed-Gaid6; Youcef Hadef7 | ||
| 1Department of Biological and Agricultural Sciences, Institute of Sciences and Technology, AbdelHafid Boussouf University Center, 43000, Mila, Algeria | ||
| 2Pharmacognosy Laboratory, Department of Pharmacy, Faculty of Medicine, University of Badji Mokhtar, 23000, Annaba, Algeria | ||
| 3Department of Molecular and Cellular Biology, Faculty of Natural and life Sciences, University of Abbes Laghrour, 40004, Khenchela, Algeria Applied Molecular and Cellular Biology, Faculty of Natural and life Sciences, University of | ||
| 4Pharmaceutical Sciences Research Center (CRSP), Constantine, 25000, Algeria | ||
| 5Laboratory of Biophysics, Faculty of Medicine, University of Badji-Mokhtar, 23000, Annaba, Algeria | ||
| 6Public Health Institute of Mohamed Layachi, 8000, Bechar, Algeria Department of Medicine, Faculty of Medicine, University of Badji Mokhtar, 23000, Annaba, Algeria | ||
| 7Pharmacy Analysis Departemnt, Medecine Faculty, 23000, Annaba, Algeria. | ||
| Abstract | ||
| This study aims to investigate the phenolic profile, antioxidant, and antibacterial activities of the methanolic extract of Haloxylon scoparium Pomel, an endemic plant of the Algerian Sahara. Quantitative analysis revealed total phenolic content of 54.77 mg GAE/g DM, flavonoids at 9.39 mg CE/g DM, and total tannins at 5.36 mg TAE/g DM. High-Performance Liquid Chromatography identified several phenolic compounds, including chlorogenic acid, caffeic acid, quercetin, catechin, rutin, and kaempferol. Antioxidant activity, assessed using the DPPH radical scavenging assay, demonstrated a strong activity with an IC50 value of 68.64 µg/ml. The methanolic extract exhibited significant antibacterial activity against various pathogenic bacteria, including Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, and Klebsiella pneumoniae, with inhibition zones ranging from 19.00 to 22.10 mm. The extract also showed promising activity against two multidrug-resistant strains. These findings support the potential use of H. scoparium as a valuable source of bioactive compounds, offering significant antibacterial properties that could be further explored for the development of alternative therapeutic agents. | ||
| Keywords | ||
| Haloxylon scoparium; methanolic extract; HPLC analysis; antioxidant; antibacterial | ||
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