Biogenesis of Silver Nanoparticles Feasible for Industrial Application Using Yeast-fungi Secreted Enzymes and Proteins | ||
| Egyptian Journal of Chemistry | ||
| Article 5, Volume 60, Conference Issue (The 8th International Conference of The Textile Research Division (ICTRD 2017), National Research Centre, Cairo 12622, Egypt.), December 2017, Pages 49-61 PDF (1.15 M) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/ejchem.2017.1623.1136 | ||
| Authors | ||
| Asem A. Mohamed* 1; M.K. Zahran2; M.H. El-Rafie3 | ||
| 1Chemistry of Natural and Microbial products department, National Research Center, Dokki, Cairo, Egypt | ||
| 2Department of Chemistry, Faculty of Science, Helwan University, Ain-Helwan, Cairo, Egypt | ||
| 3Textile Research Division, Dokki, Cairo, Egypt. National Research Center, | ||
| Abstract | ||
| Saccharomyces cerevisiae, Rhodotorula glutinis and Geotrichum candidum were selected for biosgenesis of silver nanoparticles (AgNPs) using yeast reductases. Extracellular proteins act as stabilizers for so formed AgNPs. Factors affecting biosynthesis ; biomass concentration, AgNO3 concentration and the ratio of AgNO3: to biomass concentration on. The Optimum conditions for biosynthesis of AgNPs could be attained using biomass of Saccharomyces cerevisiae (3 mL/100 mL): .9 mM AgNO3: pH 12: 25 ◦C and 24 h incubation). Under these conditions, the maximum concentration of well stabilized AgNPs obtained was 900 ppm with a mean diameter range of 1.5–12 nm. Such solution is unequivocally feasible for industrial applications. | ||
| Keywords | ||
| Silver nano particles; yeast fungi; reductase enzymes; Optimization; textile treatment | ||
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