IMPROVED MICRO PROPAGATION OF Cichorium intybus L. FROM LEAF EXPLANT | ||
| Journal of Productivity and Development | ||
| Article 2, Volume 20, Issue 2, April 2015, Pages 139-148 PDF (406.33 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/jpd.2015.42778 | ||
| Authors | ||
| Heba Shahin* 1; Yasseen Mohamed Yasseen2; Mohamed Fahim2 | ||
| 1Genetic Engineering and Biotechnology Research Institute (GEBRI), Sadat City University, , P.O. Box 79/22857, Egypt. | ||
| 2Genetic Engineering and Biotechnology Research Institute (GEBRI), Sadat City University, Sadat City, P.O. Box 79/22857, Egypt. | ||
| Abstract | ||
| Cichorium intybus is edible, medicinal and forage plant. The pharmaceutical raw materials were obtained from wild chicory (var. Maurane). Currently, farmers are increasingly assuming plantations of wild chicory, and breeders are attempting to produce cultivars for medicinal purposes. In the modern breeding of chicory important feature is the ability to propagate in vitro culture. The aim of our study was to assess capacity of natural population of wild chicory for plant regeneration from leaf explants. In the first was examined the effect of various concentrations of BA and TDZ on the regeneration of shoots from leaf explants (0.5 cm2). After that, 25 plants were propagated on the medium which was found as optimal. Then, their callus growth and shoots regeneration capacities were compared. The majority of the shoots were regenerated from callus but direct organogenesis was also observed (8%). Shoot regeneration was found to be the most efficient on MS medium containing 5MµBA and 3MµTDZ –97% of the explants produced shoots, while the average number of shoots was 15.5. The wild chicory shoots rooted easily. | ||
| Keywords | ||
| Cichorium intybus; Regeneration; TDZ; BA; callus formation and 2; 4-D | ||
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