DEVELOPMENT OF HAPLOID PLANTS THROUGH ANTHER CULTURE TECHNIQUE IN WHEAT ( Triticum aestivum L. ) | ||
| Journal of Productivity and Development | ||
| Article 3, Volume 20, Issue 3, August 2015, Pages 285-296 PDF (672.65 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/jpd.2015.42802 | ||
| Authors | ||
| Haron Abo Shama* ; Hala Ghareeb | ||
| Plant Biotech Dept . Genet. Engin. and Biotech .Res. Inst ( GEBRI), Sadat City, University Menufiya, Egypt. | ||
| Abstract | ||
| Anther culture was utilized to develop haploid plants in wheat .This study was carried out at Plant Biotechnology Department, Genetic Engineering and Biotechnology Research Institute ( G E B R I ) from 2009 to 2014 years . Three varieties of wheat were used , Sids1, Gemmeiza 9 and Giza 168. The studied factors were, cold and chemical pretreatments, growth regulators concentrations of 2,4-D and kinetin and effect of source of spikes . The results indicated that the cold pretreatment had no effect on anther response of three wheat varieties, the chemical pretreatments by sterilization substances (ethanol alcohol 70 % or mercurec chloride 1g /L or thiourea 2 g /L ) were very important and essential to induce callus from anther . The three treatments of ( 1mg /L 2,4-D ) , ( 2 mg /L 2,4-D ) and ( 2 mg /L 2,4-D + 0.1 mg /L kinetin ) gave the highest response of callus induction and plant regeneration . Androgenesis improved with increasing the cultured area which the spikes were collected from it . | ||
| Keywords | ||
| Wheat; Anther culture; Cold & chemical pretreatments; growth regulators; Effect of source of spikes; varieties | ||
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