M. A. Hussein, M., H. Hassan, W., O. Kamel, M. (2017). Pathogenicity of Aeromonas hydrophila isolated from diseased sharp teeth catfish, Clarias gariepinus, with special reference to the lethality of its extracellular products. test, 24(1), 114-123. doi: 10.21608/jvmr.2017.43271
Mortada M. A. Hussein; Walid H. Hassan; Mohamed O. Kamel. "Pathogenicity of Aeromonas hydrophila isolated from diseased sharp teeth catfish, Clarias gariepinus, with special reference to the lethality of its extracellular products". test, 24, 1, 2017, 114-123. doi: 10.21608/jvmr.2017.43271
M. A. Hussein, M., H. Hassan, W., O. Kamel, M. (2017). 'Pathogenicity of Aeromonas hydrophila isolated from diseased sharp teeth catfish, Clarias gariepinus, with special reference to the lethality of its extracellular products', test, 24(1), pp. 114-123. doi: 10.21608/jvmr.2017.43271
M. A. Hussein, M., H. Hassan, W., O. Kamel, M. Pathogenicity of Aeromonas hydrophila isolated from diseased sharp teeth catfish, Clarias gariepinus, with special reference to the lethality of its extracellular products. test, 2017; 24(1): 114-123. doi: 10.21608/jvmr.2017.43271

Pathogenicity of Aeromonas hydrophila isolated from diseased sharp teeth catfish, Clarias gariepinus, with special reference to the lethality of its extracellular products

Journal of Veterinary Medical Research
Article 12, Volume 24, Issue 1, June 2017, Pages 114-123    PDF (542.54 K)
Document Type: Original Article
DOI: 10.21608/jvmr.2017.43271
Authors
Mortada M. A. Hussein1; Walid H. Hassan2; Mohamed O. Kamel3
1Fish Department, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef 12452, Egypt. 2
2Bacteriology, Mycology and Immunology Department, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef 12452, Egypt.
3Fish Department, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef 12452, Egypt.
Abstract
0px; "> A study was conducted to investigate the pathogenicity and the median lethal dose (LD50) of Aeromonas hydrophila isolated from clinically diseased catfish against apparently healthy homologous fish to evaluate the lethality of extra-cellular products (ECPs) of the isolated strain in vivo. For pathogenicity experiment, five different concentrations of Aeromonas hydrophila strain BNS 01614 including 3× 108, 1.5 × 108, 1.5 × 107, 1.5 × 106 and 1.5 × 105 CFU/fish used via intra peritoneal. The results revealed that pathogenicity of BNS 01614 was confirmed by the mortality of 30 % to 100 % of all tested fish within 4 to 12 days with LD50 1.5 × 107 CFU/fish. The Concentrated extracellular products (ECPs) of the selected bacterium were prepared and confirmed to be toxic in Clarias garipineus with LC50 of 20µg.
Keywords
Aeromonas hydrophila; catfish; LD 50; pathogenicity
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