Bakou, S., Guigand, L., Apreutese, R., Apreutese, A., Wyers, M., Cherel, Y. (2014). Myocytes changes and satellite cells proliferation following denervation of ALD (Anterior Latissimus Dorsi) and PLD (Posterior Latissimus Dorsi) muscles in two strains of turkey (Meleagris gallopavo). test, 7(1), 89-111. doi: 10.21608/jva.2014.44718
S. Bakou; L. Guigand; R. Apreutese; A. Apreutese; M. Wyers; Y. Cherel. "Myocytes changes and satellite cells proliferation following denervation of ALD (Anterior Latissimus Dorsi) and PLD (Posterior Latissimus Dorsi) muscles in two strains of turkey (Meleagris gallopavo)". test, 7, 1, 2014, 89-111. doi: 10.21608/jva.2014.44718
Bakou, S., Guigand, L., Apreutese, R., Apreutese, A., Wyers, M., Cherel, Y. (2014). 'Myocytes changes and satellite cells proliferation following denervation of ALD (Anterior Latissimus Dorsi) and PLD (Posterior Latissimus Dorsi) muscles in two strains of turkey (Meleagris gallopavo)', test, 7(1), pp. 89-111. doi: 10.21608/jva.2014.44718
Bakou, S., Guigand, L., Apreutese, R., Apreutese, A., Wyers, M., Cherel, Y. Myocytes changes and satellite cells proliferation following denervation of ALD (Anterior Latissimus Dorsi) and PLD (Posterior Latissimus Dorsi) muscles in two strains of turkey (Meleagris gallopavo). test, 2014; 7(1): 89-111. doi: 10.21608/jva.2014.44718

Myocytes changes and satellite cells proliferation following denervation of ALD (Anterior Latissimus Dorsi) and PLD (Posterior Latissimus Dorsi) muscles in two strains of turkey (Meleagris gallopavo)

Journal of Veterinary Anatomy
Article 8, Volume 7, Issue 1, April 2014, Pages 89-111    PDF (1.28 M)
Document Type: Original Article
DOI: 10.21608/jva.2014.44718
Authors
S. Bakou1; L. Guigand2; R. Apreutese2; A. Apreutese2; M. Wyers2; Y. Cherel2
1Service d’Anatomie et Histologie – Embryologie. École Inter-Etats des Sciences Mé- decine Vétérinaires (E.I.S.M.V.) B.P. 5077 Dakar, Senegal
2UMR 703 INRA /Oniris, Nantes-Atlantic National College of Veterinay Medicine, Food Science and Engineering, CS 40706, F-44307 Nantes Cedex 03, France
Abstract
Morphological features and chronology of myocytes changes after denervation were studied over 35 days period in 2 heavy (HW) and light-weight (LW) strains of 5-week- old male turkeys. Denervation caused progressive atrophy in pos- terior latissimus dorsi (PLD). By day 28, the weight of the PLD muscle had reached about 62% of the non-denervated contralateral muscle weight in both strains. On the contrary, ALD muscle mass increase progressively after denervation. Thus the maximum hypertrophy of the ALD, expressed as a percentage of contralateral muscle, was respectively about 67% and 37%, day 21 in the HW strain and day 28 in LW strain after denervation. ALD hypertrophy ceased apparently after day 21 (HW strain) and day 28 (LW strain). Morphometric analysis revealed that fast twitch (type II) fibers were atrophied after denervation, whereas slow tonic (type III) fibers were hypertrophied from day 7. Coagulative necrosis with fragmentation and lysis associated with moderate infiltration of inflammatory cells, were similar in both strains. Irregularities in mitochondrial distribution occurred mainly in type III fibers of ALD muscle at day 7. Seven and 14 days after denervation, immunolabelling of proliferating cell nuclear antigen (PCNA) revealed satellite cell activation in denervated muscles. The number of activated satellite cells was greater in the LW than HW mainly in ALD muscles.
Keywords
skeletal muscle; fiber size; denervation; satellite cells; turkey
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