EFFECTS OF DIET ON HYDROGEN CONCENTRATION ION'S OF VAGINAL MILIEU STUDY IN THERAPEUTIC NUTRITION

EFFECTS OF DIET ON HYDROGEN CONCENTRATION ION'S OF VAGINAL MILIEU STUDY IN THERAPEUTIC NUTRITION

 

Tarek Abd El-Rahman

Department of Nutrition and Food Sciences, Faculty of Home Economics, Minufiya University, Shebin El-Kom, Egypt.

 

ABSTRACT

Diet plays an important role for equilibrium the values between acidic and basic levels in the human body which expressed as ion's hydrogen concentration. In this study fifty volunteer mature females initially participated. The female's age ranged from 18 to 36 year old. 23 females from 50 were virgins; while other 27 females were married. The pH of the vagina was assessed by the women themselves "invasively" by introduction of a special indicator paper (pH-strips). Conditions of examination: pH-strips were applied at morning before pissing for pH vaginal milieu assay. In day fourteen and for three succeeding days after of menstrual pH, diet, hormone and vagina (ecosystem) were assayed. Methods A. chemical examinations: pH vaginal milieu assay. acidifying food reaction, alkalizing food reaction. Sex hormone gonadotrophin (hCG) in urine chromatography assay and KOH test for fishy odor; B. Physical examinations: Vaginal canal temperature (VCT) C^º as indication of both vaginal infection and oxygen expenditure and specific gravity (SG) for urine; C. Hematological tests: Serum enzyme Tests as alanine aminotransferase (AST) aspartate aminotransferase (ALT) and bilirubin enzyme. D .Statistical analysis: as mean ±SD was carried out. This study results confirmed by previous investigations illustrated that diet could be affecting on pH value for vaginal milieu and could correct the vaginal discharge pH. Moreover diet could correct systemic diseases as vaginal infection. 

      Keywords: Hydrogen concentration ion's, vaginal milieu, therapeutic nutrition

 

INTRODUCTION

         Some women who have high acidity vaginal levels tend to exhibit these symptoms such as: anxiety, diarrhea, dilated pupils, extroverted behavior, fatigue in early morning, headaches, hyperactivity, hyper sexuality, insomnia, nervousness, rapid heartbeat, restless legs, shortness of breath, strong appetite, high blood pressure, warm dry hands and feet, The disturbances of the vaginal milieu or genital infections is one of the most important avoidable causes mostly combined with bacterial vaginosis (Saling et al.,2001). The pH assessment along with symptoms and signs could be used as initial screening test to alert physician to vaginitis. When vaginal fluid pH is used to screen for Bacterial vaginosis, it has a greater sensitivity than when used to screen for all causes of vaginitis.Di Renzo and De-Domenico (2000)^a,b found that Vaginal pH undergoes physiologically changes from birth to menopause, according to changes of ovarian steroids occurring during woman's life. Adequate levels of estrogens play a fundamental role in the trophism of vaginal mucosa. In fact, estrogens increase the cellular content of glycogen. Exogenous activities on vaginal pH can be exerted by several factors, such as sexual activity, oral contraceptives, systemic diseases .Melis et al. (2000) reported that the liver functions is the manufacturing of vital digestive enzymes.   Another function of the liver is processing waste. The liver is responsible for chemically adjusting the body's cell waste so that the kidneys can extract it from the blood. If this function is impaired, the body will soon poison itself. Acidosis is the tendency of the body to be over acid. This occurs when the body loses its alkaline reserve. Persons who have diabetes often suffer from acidosis. The symptoms of acidosis are insomnia, rheumatoid arthritis, migraine headaches, hard dry stools, sensitivity of teeth to vinegar and acid fruits and others. The causes of acidosis are kidney, liver, and adrenal disorder,improper diet, anger, stress, and excess niacin, vitamin C, and aspirin.  Although it might seem that citrus fruits would have an acid effect on the body, the citric acid they contain actually has an alkaline effect in the system, converting to carbon dioxide and water(Melis et al., 2000). Alkalosis occurs when the body is too alkaline. It is often the result of excessive intake of alkaline drugs such as sodium bicarbonate for the treatment of gastritis or peptic ulcers. It can result in high cholesterol, endocrine imbalance and osteoarthritis. The symptoms of alkalosis include creaking joints, bursitis, allergies, chronic indigestion and menstrual problems. Alkalosis occurs less often than acidosis because our Western diet is predominantly acid-forming. Alkalosis may cause calcium to build up in the body, such as in bone or heel spurs (Anon 2000). Therefore the present study was done to cleared role of diet on hydrogen concentration on vaginal milieu.

 

SUBJECTS AND METHODS

A. Sample:

Fifty volunteer mature females initially participated in this study age ranged from 18 to 36 years. Twenty three of subjects were virgins, while the rest were marred.

B. Examination conditions:

Examinations were carried out in the morning before pissing to determine the pH of vaginal milieu. In the 14^th  menstrual day and for 3days after menstruation. The vaginal pH, diet, hormone and vagina ecosystem parameters were assayed.

C. Methods:

C .a .Chemicals tests:

C .a .1. Vaginal milieu pH

It was assayed right after attention (wake up) and before passing in control subjects group, using Nitrazine paper kit according to Harstall, and Corabian, (1998).

C .a .2.Acidifying food reaction:

100 gm milk +100 gm boiled beef were taken right after attention and prior to pissing to assay the acidifying effect of food reaction in 15^th day of menstruation. Determination was carried out 3 hours after food consumption.

C .a .3.Alkalizing food reaction:

250 g natural orange organic juice were taken right after attention time after passing for assay alkalizing food reaction effect .Vaginal  pH assay was carried out in  the 16^th day of menstruation, 3 hours after food consumption       ( Barooty, 1991).

C .a .4. Sex hormone gonadotrophin (hCG) in urine:

hCG hormone was determined in urine using chromatography paper kit assay   method according to Chessbrough ,Monica( 2000).

C .a .5. Potassium hydroxide Test (KOH test):

KOH test for fishy odor assay was carried out according to method described by Doty et al. (1975).

C.b. Physicals tests:

C.b. 1.Vaginal temperature:

The temperature of vagina (in ºC) is used as   indication for vaginal infection as well as oxygen expenditure consumption. This was carried out according to Cheesbrough ,Monica ( 2000).

C.b.2.Urine collection.

Area around the urinary aperture was cleaned with water, dried then  the individual urinated (pissed) in a sterilized wide glass container, which was rapidly discharged in a sterilized glass bottle.

C.b. 3. Specific gravity ( SG):

SG of urine was determined using the viscosemeter as recommended by Wallach (1978) and Tietz (1986).

C.C. Serum tests:

C.C.1. Separation of blood serum:

The serum was separeated from the whole blood according to the method described by Chessbrough, Monica( 2000).

C.C.2. Serum Enzymes :

Alanine aminotransferase (AST) and aspartate aminotransferase (ALT) according to the methods published by Reitman and Frankel (1957).

C.C.3. Bilirubin of serum:

Bilirubin was determined in serum by direct colorimetric method, as described by Jendrassik  (2000).

D- Statistical analysis:

Statistical analysis was carried out via calculation of mean values and standard deviation (±SD) according to Normain and Baily(1997).

 

RESULTS AND DISSECTIONS

 

1-Examination of Virgin females of the control group:

Data of Table (1) show the chemical, physical and serum examinations carried out for virgin females. Such results provide precise in formation considered as standard measurements to characteristic the control group as reported by Rashad et al. (1992). From results of Table 1, it could be noticed that mean pH value was (3.79±0.55)  which fell in the normal vaginal pH of healthy virgins as reported by Falngas et al. (1997); Poulard et al. (1997); Moats & Maddox (1998) and Chessbrough, Monica (2000). The  exception was recorded for case 22 which showed a higher more alkaline pH of (5.5) indicating suffering of vaginal infection as explained by Brizzolasa et al.(1998), Di Renzo and De Domenico (2000)^a,b and Melis et al. (2000).

The data of Table (1) scoped light on human chronic gonadotrophin (LCG).Such hormone which is a glycoprotein secreted by developing placenta post fertilization, show rapid increased concentration in urine of the pregnant women was observed.  Detection of hCG is an excellent early marker confirming pregnancy or predicting the hormonal disorder for the woman to obtain definite confirmable results hCG should be noticed less than 100ml u/ml. From result (Healthing and pregnant females) for all cases according to Simpson and MacDonald (1981) and Mitreski. (2002),who recommended to carry out hCG test for virgins (as well as the married) in comparison studies, since hCG abnormality is possible and may indicate false pregnancy for the virgins.

Vaginal canal temperatures were measured and data presented in  Table 1. The results revealed that all cases were at normal vaginal canal temperature of 37.3 ^ºC. According to Rashad et al. (1992); Anon(2000) and Juarezs et al. (2002) the range of vaginal canal temperature for normal healthy females is 37.3-37.4 ^ºC. The case No.(22) represent an exception showing higher vaginal canal temperature of 37.5 ^ºC, which could be resulting from vaginal canal infection as reported by Balzarini et al. (1998) and Chessbrough, Monica (2000).

Table 1: The chemical, physical andserum examinations of virgin females as control group.

Case  No.	Age	pH	HCG mlU/ml	Vaginal Temperature Cº	SG	AST μ/L	ALT μ/L	Bilirubin μmol/L	KOH
1	18	3	N	37.3	1.03	12.68	9.11	13.26	N
2	18	3.5	N	37.3	1.03	19.01	9.11	6.30	N
3	18	4.2	N	37.3	1.03	13.06	8.02	6.53	N
4	18.6	3.8	N	37.3	1.02	11.08	7.26	7.62	N
5	18.9	4	N	37.3	1.03	5.05	14.66	1.80	N
6	20	3.6	N	37.3	1.03	9.86	9.10	9.42	N
7	20	4.1	N	37.3	1.03	15.26	15.21	2.33	N
8	20.6	3.4	N	37.2	1.03	2.19	17.60	7.57	N
9	21	3.7	N	37.3	1.02	6.68	7.01	11.69	N
10	21	3.6	N	37.3	1.03	14.33	18.26	8.21	N
11	22	3.2	N	37.3	1.03	6.10	3.30	10.37	N
12	23	4.2	N	37.3	1.03	7.12	6.03	12.43	N
13	23	3.9	N	37.3	1.03	14.95	19.57	2.73	N
14	23	4.2	N	37.3	1.02	3.33	2.06	6.80	N
15	24	3.9	N	37.3	1.03	4.02	9.07	8.27	N
16	25	3.1	N	37.3	1.03	9.15	6.30	4.22	N
17	25	4.2	N	37.3	1.02	9.23	4.07	7.62	N
18	25.5	3	N	37.3	1.03	9.09	18.26	6.55	N
19	26	3.3	N	37.3	1.03	6.03	19.89	16.60	N
20	26.1	3.8	N	37.3	1.03	8.06	14.70	13.46	N
21	26.8	4.2	N	37.3	1.03	16.01	2.95	9.22	N
22	27	5.5	N	37.5	1.03	18.26	30.25	19.98	p
23	28	3.7	N	37.3	1.03	6.22	8.05	8.29	N
Mean		3.787	0	37.304	1.03	9.80	11.30	8.75	0.043
SD		0.546	0	0.047	0.00	5.22	6.99	4.40	0.209

Specific gravity was measured at temperature between 15.5 C^º and 37.8 Cº as RI.*

P positive is K OH test for vaginal fishy odor at concentrate 10%.

**N was negative result for hCG.

 

Specific gravity (SG) of urine ,measured as refractive index (RI) between 15.5^ºC and 37.8^ºC is shown in Table 1. Values were 1.02 - 1.03(mean value 1.03) , being  in  the  normal  scale  range (1.000 to 1.030) developed  by Chessbrough, Monica(2000). Reversals SG was reported by Wallach (1978) and Tietz (1986) the increased amounts of protein, glucose or radiographic contents medium in urine may result in high specific gravity values not necessarily indicative of normal renal function.

­ Alanine aminotransferase (AST μ/L) was measured and data presented in Table 1. For all cases AST values were at the normal level (mean value 9.80±5.22) μ/L, with exception of the case No.(22) where it was pronouncedly higher begin 18.26 μ/L. Nevertheless, Reitman and Frankl (1957) studied that normal AST was 2 to 20 μ/L. Chessbrough, Monica (2000) reported that very high AST activity usually accompanies liver disease specially in case of viral hepatocellular damage (infection hepatitis). In viral hepatitis, the serum or plasma AST is often raised before the patient appears jaundiced and remains elevated for as long as the virus remains active. In case of myocardial infarction AST elevated and in case of obstruction liver disease.

  Data presented in Table show the aspartate aminotransferase level (ALT μ/L), mean value was (11.30±6.99) except for case No. (22), which showed remarkably high (30.25 μ/L) value .Therefore, majority of cases (about 96%, 22 of 23 cases) the level was mostly normal, being in the rang of (2-15 μ/L given by Reitman and Frankl. (1957). Champe & Harvey (1994) reported the use of ALT activity as indicator to myocardial damage or tissue inflammation due to vaginal infection (Hillier et al., 1992).

  Bilirubin enzyme was determined (μ mol/L) and results illustrated in Table 1. Such results revealed that mean value was8.75 indicate a normal level which according to Jendrassik, (2000) fell in the normal rang 1.71-20.52 μ mol/L. Chessbrough, Monica (2000) and Jendrassik (2000) showed that bilirubin was formed from the breakdown of erythrocytes and other heam-containig proteins such as myoglobin and cytochromes. Anyhow it may be concluded that all studied case was for females of healthy physiology state.

     Potassium hydroxide test (KOH test) was carried out for the members of investigated virgins. For 22 of 23 cases the reaction was negative, with except for the case No.(22), where the test showed was positive reaction (fishy odor) which emphases was infected by microorganism's in vaginal canal. Such results  may be in agreement with  Yavuzdemir et al.(1992)  who  reported that 10% of studied cases reveals the "fishy odor" has been investigated infection with Gardnerella vaginalis, or Trichomonas vaginalis, various bacteria or infection with polymicrobial etiology.

2-Examination of married females of the control group:

Data of in Table 2 show the chemicals, physical and (serum hematological) examinations carried out on married females as control group. It was noticed that mean vaginal pH was (4,170±0.812) indicating normal cases (Rashad et al., 1992(, meanwhile cases No.1, 11, 16, 23 and 27 had higher values (5.5, 5.8, 5.1, 5.5, and 5.3  respectively). Briwiara et al. (1999) and Melis et al. (2000) studied that vaginal pH my be reach to alkaline reaction as results of genital infection which is common among for 70-80%.

Also, that revealed normal  hCG levels among the control married women sample, indicating no pregnancy or hormone disturbance (Frassetto et al., 2001). Vaginal canal temperature measurements (Table 2) revealed clearly that cases No. 1,11,16,23 and 27 had slightly high temperature (37.6, 37.5, 37.5, 37.6 and 37.5°C respectively, indicating which vaginal infections as reported  by  Rashad et al.  (1992);  Baharini et al. (1998);  Anon  (2000)  and

Table 2: The chemical, physical and serum examinations on married females as control group.

Case No.	Age	pH	hCG mlU/ml	Vaginal Temperature Cº	SG	AST μ/L	ALT μ/L	Bilirubin μ mol/L	KOH
1	27	5.5	N	37.6	1.036	16.325	21.169	22.085	p
2	27	4.2	N	37.3	1.026	6.099	14.222	13.049	N
3	27.9	4.4	N	37.3	1.028	7.121	15.369	17.065	N
4	28	3.9	N	37.3	1.025	14.954	6.257	19.052	N
5	28	3.2	N	37.3	1.039	3.332	11.848	20.020	N
6	28	4.3	N	37.3	1.025	4.022	12.680	15.066	N
7	28	5.6	N	37.3	1.030	9.155	17.881	18.030	N
8	28.7	3.5	N	37.3	1.023	9.230	13.555	17.025	N
9	29	3.2	N	37.3	1.025	9.089	8.126	15.068	N
10	30	4.1	N	37.3	1.023	6.026	19.250	17.025	N
11	30	5.8	N	37.5	1.035	16.059	30.655	23.085	p
12	30.11	3.9	N	37.3	1.024	9.015	18.246	11.244	N
13	31	3.5	N	37.3	1.024	10.256	8.247	8.040	N
14	31	3.7	N	37.3	1.029	6.215	6.299	13.024	N
15	31	3.2	N	37.3	1.023	14.954	19.258	19.066	N
16	32	5.1	N	37.5	1.039	18.233	25.477	23.084	p
17	32	3.7	N	37.3	1.027	4.022	16.783	3.052	N
18	32	4.2	N	37.3	1.030	9.155	8.259	14.035	N
19	34	3.8	N	37.3	1.030	6.099	9.215	5.042	N
20	34	4.6	N	37.3	1.028	7.121	11.256	9.054	N
21	34	3.6	N	37.3	1.026	14.954	4.026	20.070	N
22	35	4.2	N	37.3	1.027	3.332	9.126	14.026	N
23	35	5.5	N	37.6	1.039	17.498	23.655	24.085	p
24	36	3.1	N	37.3	1.024	9.155	8.245	7.030	N
25	36	3.4	N	37.3	1.030	9.230	7.564	16.071	N
26	37	4.1	N	37.3	1.024	9.089	18.216	6.049	N
27	37.11	5.3	N	37.5	1.033	17.959	26.219	22.088	p
Mean		4.170	0	37.344	1.029	9.915	14.485	15.246	0.185
SD		0.812	0	0.097	0.005	4.727	6.981	5.922	0.396

Specific gravity was measured at temperature between 15.5 C^º and 37.8 Cº as RI.*

Ppositive is K OH test for vaginal fishy odor at concentrate 10%.  .                                    

**N was negative result for hCG.

 

Chessbrough, Monica (2000);others women had normal vaginal temperature level (37.344°C±0.097).

Specific gravity (S.G) of urine measured between 15.5 °C and 37.8 °C as   refractive  index  (RI) ,  showed  normal  range  scale  1.000  to  1.030 (mean ±SD 1.029± 0.005(for most of sample subjects (Table 2).Nevertheless higher values were raised for cases No. 1,11,16,23 and 27; being 1.036, 1.035, 1.039, 1.039and 1.033 respectively. This was in according with findings reported by Wallach, (1978) and Tietz, (1986).

Aspartate aminotransferase (ALT µ/L) values in (Table 2) showed that studies (81.5) was normal level (mean ± SD values was 9.915±4.727). While, in 18.5% of cases (values were high which were (16.325, 16.059, 18.233, 17.498and 17.959) for cases No. l, 11, 16, 23 and 27 respectively meanwhile were also within normal range according to Reitman and Frankl (1957). In the same Table 2, alanine minotransferase(ALT µ/L) activity averaged (14.485±6.981) indicating that most of control married women were at normal level, with except for cases No 1.11,16,23 and 27(which had also higher ALT than normal) which expressed abnormal high values: being (21.169, 30.655, 25.477, 23.655 and 26.219 respectively) .As reported by Champe and Harvey(1994)ALT used as indicator to myocardial damage or tissue inflammation. That value was indicated to inflammation due to vaginal infection Hillier       et al. (1992).

Bilirubin (µmol/L) was determined and data presented in Table 2 revealed clearly that women of tested group were normal (mean ± SD was 15.246±5-922, while cases No. 1, 11, 16. 23 and 27 (22.085, 23.085, 23.084, 24.085and 22.088 respectively) had higher levels indicating inflammation due to vaginal infection .Chessbrough, Monica (2000) studied that high value of bilirubin together with raised aminotransferase level could be found before a patient became clinically jaundiced.

The value of potassium hydroxides (KOH) test (Table 2) most of control married women were of negative reaction indicating the vaginal infection; this was also deduced from other studied parameters as presented in Table 2  for mentioned cases.

3-Examination of vaginal milieu for virgin females as affected consuming by the acidifying reaction food:

Data in Table 3 indicated that  pH of vaginal milieu for virgin females under acidifying food  reaction (100 g Milk +100 g boiled beef) which were eaten at soon attention time after (until avoiding other food interaction probably was resulting from dinner meal) passing for assay acidifying food reaction in day after of menstrual, pH was assay after 3 hours. The results illustrated the pH value was in normal range the mean± SD was (3.4±0.5) data obviously the hydrogen power ion's tend to be more acidity as reactions for acidifying food reaction Yasin et al. (2002) except for case No. (22) Which had highly level tended to be inside alkalosis pH was (4.9). A comparison between both levels for the same case No.(22), when at normal diet and acidifying diet data obviously pH was (5.5 and 4.9 respectively) this result raveled that acidifying food could effect on vaginal milieu towards reduced alkaloses of vaginal milieu .the results agree with Doty et al. )1975); Goldfoot,

 

Table 3: PH of vaginal milieu for virgin females under act acidifying food reaction (100 g Milk +100 g boiled beef) which were eaten at soon attention time after passing for assay acidifying food reaction in day after of menstrual, pH was assay after 3 hours

 *  Specific gravity was measured at temperature between 15.5 C^º and 37.8 Cº as RI.

*  N wasNegative K OH test for vaginal fishy odor at concentrate 10%.

 

(1981); Russell et al. (1980); Wallis (1986); Doty (1981); Kreutz and Ackermann (1996); Stiasny et al. (1996) and Bui et al. (1996).

Vaginal canal temperature was measurement and data in Table 3 explained that all cases studies at normal vaginal canal temperature (37.3 °C) mean ±SD was (37.3±0.0) compare with case No. (22) in Table 1, which had highly level of vaginal temperature and positive for K.OH test it was indicator for surfing from vaginal infection, data in Table (3) also spotlight on correction for vaginal pH and case have normal vaginal temperature and negative for KOH test in this study, this result revealed acidifying food had correction for vaginal milieu and infection, data was agreement with those Reynolds (1995); Ferris (1997)  and Sobel. (1996).

Specific gravity (S.G) was measured at temperature between 15.5 °C and 37.8 "C as refractive index (RI), the was tabulated in Table 3 clearly that S.G was at normal range scale 1.000 to 1.030 mean ±SD was (1.0275±0.002482) Chessbrough, Monica (2000), While case No.(22) become within normal value reach to (1.0291) compare its case in Table (1) which was (1.0310) so data illustrated possibility to correct Specific gravity (S.G) in this study, data agree with Wallach, (1978) and Tietz, (1986).

Potassium hydroxides (KOH), also was assayed and data illustrated (Table 3) that alt cases studies have negative reaction and mean ±SD was         (0±0.), data emphases that acidifying food can correct virginal infection as compare the same test in Table 1, which have positive test for case No. (22) As infected and gave fishy odor. Data agree with Smeltzer and Whipple (1991) and Moraes. (1998).

 

4- Examination of vaginal milieu for married females as affected by consuming the acidifying reaction food:

Data in Table 4 show pH vaginal milieu for married females under act acidifying food reaction (100 g milk +100 g boiled beef), which were eaten at soon attention time after passing for assay acidifying food reaction in fifteen day after of menstrual, pH was assay after 3 hours. Results (Table 4) illustrated the pH value was in normal range the mean± SD was (4.01±0.69) that mentioned to almost patient was normal Rashad et al. (1992),some cases had values tend to be alkaline pH as case No. 1,11, 16, 23 and 27 which was values(5.0. 5.3. 4.6. 5.0 and 4.8 respectively), and acidifying food was lead lo reducing slightly vaginal pH towards acidity compare with same cases study at normal diet pH reach to 5.5, 5.8, 5.1 ,5.5 ,and 5.3 respectively, data agree with McClintock (I971); Kreutz & Ackermann (1996); Stiasny et al. (1996); Bui   et al. (1996); Glomb-Reinmund & Kielian (1998); Ramsey et al. (2002); Yasin et al. (2002) and Ready et al. (2002).

Vaginal' canal temperature (Table 4) revealed that all cases study at normal vaginal canal temperature (37.3 °C) mean ±SD was (37.3+1.30) compare with results in Table (2) which tent to wards elevated temperature especially cases (1.11,16,23 and 27), reach to 37.6, 37.5, 37.5, 37.6 and 37.5 °C, respectively, data clarify acidifying food could correct vaginal canal temperature in present study, the results agree with that obtained by Rashad et al. (I992) Balwini et al.(1998); Anon (2000); Chessbrough, Monica (2000) and Brody et al. (2002).

Specific gravity (S.G) was measured at temperature between 15-5 °C and 37.8 °C as refractive index (RI), (Table 4) clearly that S.G was inducing normal range (scale 1.000 to 1.030) mean ±SD was (l.0287±0,0052) except for cases No.11. 16, 23 and  27 which reach to 1.0355, 1.0393, 1.0392 and 1.0336 respectively, compare with Table 2. Al contra indicator cases No. (1, 11.16,23 and 27) was have high level reach to (1.0360. 1.0351. 1.0389, 1.0388 and

Table 4: pH vaginal milieu for married females under act acidifying food reaction (100 g milk +100 g boiled beef) which were eaten  at soon attention time after passing for assay acidifying food  reaction in fifteen day after menstrual, pH was assay after 3 hours.

  Specific gravity was measured at temperature between 15.5 C^º and 37.8 Cº as RI.*

  P^* Positive is K OH test for vaginal fishy odor at concentrate 10%

  L*is low fishy vaginal odor.

 

 

 

 

 

 

1.0332 respectively) So, data emphases obviously correct resulting from consuming acidifying food for S.G .the result agree with Wallach, (1978), Tietz, (1986), Emancipator (1992) and Simpson and MacDonald( 1981).

Potassium hydroxides (KOH) in Table 4 showed that all cases studies inducing normal values and mean ±SD was (0.11±0), except for cases No.(11,16,23 and 27) which reach to 1.0355, 1.0393, 1.0392 and 1.0336 respectively, but this value lower compare with Table 2. Potassium hydroxides was (1.0360, 1.0351, 1.0389 and 1.0388, respectively) the data illustrated enhancement in result value for Potassium hydroxides in this study especially case number (1) in Table 4, it would be correct, data agree with Horowitz (1991); Sobel (1996); Williams. (1997);   Culver (1997) and Moraes (1998).

 

5- Examination of vaginal milieu for virgin females as affected by consuming the alkalizing reaction food:

In Table 5, pH vaginal milieu for virgin females under act alkalizing food reaction 250 g fresh organic orange juice which were drink at soon attention time after passing for assay alkalizing food reaction in sixteen day after menstrual, pH was assay after 3 hours. The results illustrated the pH value was in normal range the mean± SD was (4.13±0.95) and results value tent to be towards alkaloses especially case No. (22) which reach to (4.8) .the result compare with data in Table 1, which reach to mean± SD was (3.787±0.546), data investigated the effect of alkalizing food  reaction on alternative vaginal milieu to alkaline in present study, and results agree with those obtained by Moore et al. (1988); Hrowitz (1991); Barooty (1991); Stubbs_el al. (1991); Cousins (1992); Greenberg (1996); White  and Wilson (1996); Moraes (1998); Sobel (1996); Williams (1997); Culver (1997); Bishop and Anderson (I997); Goldberg (1998), and Northrup (2000). Vaginal canal temperature indicating that all cases study inducing normal value (37.3 °C) mean ±SD was (37.295±0.023), results agree with Chessbrough, Monica (2000).

Specific gravity (S.G) was measured between 15.5 °C and 37.8 °C as refractive index (R.I). the was tabulated in Table 5 emphases that S.G was at normal range (scale 1.000 to 1.030) mean ±SD was (1.0292±0.0) from that value could be noticed that case No. (22) Had slightly raise level than normal which was (1.0314) compared with same case at normal diet in Table 1, which reach to (1.0310), according to reportRobert and Carl (2003) in an adult, about 1 liter of blood per minute passes through the kidneys. By executing their primary duty, the kidneys keep the blood alkaline and extract acid. Kidneys that are overstressed with too much acidity create kidney stones, which are composed of waste acid cells and mineral salts that have become gummed together in a waste acid substance. Therefore, by reducing acid-forming products from entering the body, the chances are better that you can avoid this painful condition.

Table 5: pH vaginal milieu for virgin females under act alkalizing food reaction 250 gm fresh orange organic juice which were drink at soon attention time after passing for assay alkalizing food reaction in sixteen day after of menstrual, pH was assay after 3 hours.

    Specific gravity was measured at temperature between 15.5 C^º and 37.8 C^º as RI.*

     P^* positive is K OH test for vaginal fishy odor at concentrate 10%                                                              

 

 

 

 

 

 

Potassium hydroxides (KOH) in Table 5 was assayed and data illustrated that all cases studies were normal and mean ±SD was (0.0±0.0) as compared with Table 1 mean ±SD was (0.0434±0.20851) when case No.(22)was positive. Data revealed that any harmful affect for alkaline food of vaginal

milieu which agree with that obtained by Moraes (1998); Horowitz (1991); Sobel (1996); Williams (1997) and Culver (1997).

 

6- Examination of vaginal milieu for married females as affected by consuming the alkalizing reaction food consuming:

In Table 6 concerned with the pH vaginal milieu for marriage females under act alkalizing food reaction 250 Gm fresh orange organic juice which drink at soon attention time after passing for assay alkalizing food reaction in sixteen day after menstrual. pH was assay after 3 hours. pH value was in normal range the mean± SD was (4.2074±0.4358) compare with value in Table (2) which reach to mean ± SD was (4.17037±0.8123) although mean± SD for Table (2) as control was show higher value than values in (Table 6) .the result could be investigate basis on some cases in control group surfing from  vaginal  infection, data in this study emphases that alkalizing food not be harmful for marred female and lead to pH vaginal milieu to be alkalosis, data agree with Moore et al. (1988), Stubbs et al. (1991); Horowitz (1991);  Barooty (1991); Cousins (1992); Sobel (1996); Greenberg (1996); Williams (1997); Culver (1997); Bishop and Anderson (1997); Moraes (1998); Goldberg (1998) and Northrup (2000). Vaginal canal temperature was measurement and data in Table 6, explain that all cases study at normal vaginal canal temperature (37.3 °C) mean ±SD was (62.1666±89.591) results agree with Chessbrough, Monica (2000).

Specific gravity (SG) was measured at temperature between 15.5 °C and 37.8 °C as refractive index (RI) the was tabulated in Table (6) indicating that S.G was at normal range scale 1.000 to 1.030 mean ±SD was (1.0285±0.0048) although some cases show slightly marginal high level, results agree with Robert and Carl (2003).

Potassium hydroxides (KOH) was assayed in Table 6 and data illustrated that all cases studies have negative reaction and mean ±SD was (0.0±0.0) compare with Table 2 mean ± SD was (1.0285±0.0050) when case number ((1, 11, 16, 23 and 27) which had positive reaction, data results agree with Hillier et al. (1992); James et al. (1992) Yavuzdemir et al. (1992) and Chessbrough, Monica (2000).

Conclusively, This study results confirmed by previous investigations illustrated that diet could be affecting on pH value for vaginal milieu and could correct the vaginal discharge pH. Moreover diet could correct systemic diseases as vaginal infection. Also, recommended eating alkaline food reaction to maintenance of health.

 

Table 6: pH vaginal milieu for marriage females under act alkalizing food reaction 250 gm fresh orange organic Juice which drink at soon attention time after passing for assay alkalizing food reaction in sixteen day after of menstrual, pH was assay after 3 hours.

  Specific gravity was measured at temperature between 15.5 C^º and 37.8 Cº as RI.*

  P^* positive is K OH test for vaginal fishy odor at concentrate 10%

 

 

 

 

 

Acknowledgment

Author is given his acknowledgment for physician of gynecology Dr. Fekry El-Hossini. Counselor Gynecologist in Ministry of Health and Population for kind and valuable help for take samples of study.

 

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تأثیر النظام الغذائی على ترکیز أیون الهیدروجین للوسط البیئى للمهبل

- دراسه فى التغذیه العلاجیه-

 

طارق عبد الرحمن                            

قسم التغذیة وعلوم الأطعمة- کلیة اﻹقتصاد المنزلى-جامعة المنوفیه-مصر

 

 

 

           یلعب الغذاء دورا هاما فى اتزان القیمه الحامضیه والقاعدیه فى جسم الأنسان والذى یعبرعنه بترکیز الأیون الهیدروجینى.اجریت الدراسه الحالیه على خمسون متطوعه من الأناث البالغات اعمارهن تتراوح بین 18-36 عاما وکان 23 منهن کن عذراوات بینما  الأخریات کن متزوجات وتم تقدیر الس الهیدروجینى لهن بواسطة شریط قیاسى ورقى استخدمنه بانفسهن صباحا قبل التبول وذلک لتقدیر الوسط المهبلى وفى الیوم الرابع عشر ولمدة 3 أیام متتابعه بعد الحیض تم التقدیر الکیمیائى للأس الهیدروجینى للحامضیه والقاعدیه الغذائیه والهرمون الجنسى جونادوتروبین فى البول طیفیا وقدر اختبار هیدروکسید البوتاسیوم (الرائحه السمکیه)واجریت الأختبارات الطبیعیه لحرارة قناة المهبل للدلاله على العدوى المهبلیه واستنفاذ الکسجین وقدرت الکثافه النوعیه فى البول.اجریت دراسه هیماتولوجیه قدرت فیها انزیمى الأسبارتات والألانین ناقلى الأمین وقدر البلیروبین.دلت نتائج الدراسه على ان الغذاء یؤثر على قیمة الأس الهیدروجینى للوسط المهبلى بل تعدى الى تصحیح الدینامیکیه المرضیه لعدوى المهبل.

التوصیة :دلت نتائج الدراسه الحالیه على ان الغذاء یؤثر على قیمة الأس الهیدروجینى للوسط المهبلى بل تعدى الى تصحیح الدینامیکیه المرضیه لعدوى المهبل، کما توصى الدراسه بتناول الطعام قلوى التاثیر للحفاظ على الصحه.