Immunohistochemical, cellular localization and expression of inhibin hormone in the buffalo (Bubalus bubalis) adenohypophysis at different ages | ||
| Journal of Veterinary Anatomy | ||
| Article 7, Volume 5, Issue 2, October 2012, Pages 83-104 PDF (1.45 M) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/jva.2012.44876 | ||
| Authors | ||
| H. F. Attia1; M. M. Kandiel2; T. A. Ismail3; M. M. Soliman4; M. A. Nassan5; A. A. Mansour6 | ||
| 1Department of Histology and Cytology, Faculty of Veterinary Medicine, Benha University, Egypt. | ||
| 2Department of Theriogenology, Faculty of Veterinary Medicine, Benha University, Egypt. | ||
| 3Department of Physiology, Faculty of Veterinary Medicine, Zagazig University, Egypt & Department of Medical Laboratories and Medical Biotechnology, Faculty of Applied Medical sciences, Taif University, Turabuha branch, KSA. | ||
| 4Department of Biochemistry, Faculty of Veterinary Medicine, Benha University, Egypt. | ||
| 5Department of Pathology, Faculty of Veterinary Medicine, Zagazig University, | ||
| 6Department of Genetics, Faculty of Agriculture, Ain Shams University, Egypt. | ||
| Abstract | ||
| The pituitary adenohypophysis was obtained from thirty buffaloes-cows, their age's ranges from one month to12 years. Sections of adenohypophysis tissues were immunostained for α, βa, and βb subunits of inhibin hormone. Positive immuno-staining specific for the α subunits of inhibin were detected in the cells of the follicles of the adenohypophysis in all ages. Moreover, immunostaining specific for the inhibin βa subu- nits were strong positive at one month, weak positive at 4.5, 8 and 12 years and were negative at 8 months and 1.5 years. However, the immunostaining specific for the in- hibin βb subunits were positive at one and 8 months, 8 and 12 years and weak positive at 4.5 years and negative at 1.5 year. RT-PCR analysis revealed that both α and βb subunits are expressed in all ages except at 1.5 years old animals while βa subunit is only expressed at young age. | ||
| Keywords | ||
| Inhibin; Buffalo-cows; Adenohypophysis; RT-PCR; Immunohistochemistry | ||
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