Al Aiyan, A., Barigye, R., Mohamed, M., Menon, P., Hammoud, M., Richardson, K. (2018). Microbial Status of Animal Anatomical Cadavers Fixed Using Low Formaldehyde Concentrations. test, 11(2), 1-16. doi: 10.21608/jva.2018.44907
A. Al Aiyan; R. Barigye; M. E.H. Mohamed; P. Menon; M. Hammoud; K. Richardson. "Microbial Status of Animal Anatomical Cadavers Fixed Using Low Formaldehyde Concentrations". test, 11, 2, 2018, 1-16. doi: 10.21608/jva.2018.44907
Al Aiyan, A., Barigye, R., Mohamed, M., Menon, P., Hammoud, M., Richardson, K. (2018). 'Microbial Status of Animal Anatomical Cadavers Fixed Using Low Formaldehyde Concentrations', test, 11(2), pp. 1-16. doi: 10.21608/jva.2018.44907
Al Aiyan, A., Barigye, R., Mohamed, M., Menon, P., Hammoud, M., Richardson, K. Microbial Status of Animal Anatomical Cadavers Fixed Using Low Formaldehyde Concentrations. test, 2018; 11(2): 1-16. doi: 10.21608/jva.2018.44907

Microbial Status of Animal Anatomical Cadavers Fixed Using Low Formaldehyde Concentrations

Journal of Veterinary Anatomy
Article 1, Volume 11, Issue 2, October 2018, Pages 1-16    PDF (1.51 M)
Document Type: Original Article
DOI: 10.21608/jva.2018.44907
Authors
A. Al Aiyan* 1; R. Barigye1; M. E.H. Mohamed1; P. Menon1; M. Hammoud2; K. Richardson3
1Department of Veterinary Medicine, College of Food and Agriculture, United Arab Emir- ates University, Al Ain, UAE
21Department of Veterinary Medicine, College of Food and Agriculture, United Arab Emir- ates University, Al Ain, UAE
3College of Veterinary Medicine, School of Veterinary and Life Sciences, Murdoch Uni- versity, Perth, Australia
Abstract
Microbial growth on the skin and organ surfaces is a common problem associated with formaldehyde fixation of animal cadavers and is especially so following watering of cadavers prior to dissection. Cadavers of three sheep, one goat and three horses were fixed with a solution of 2% formaldehyde, 30% ethanol, and 20% polyethylene glycol 400. At time intervals tissue samples of; skeletal muscle, lung and intestine were analyzed for cultivable aerobic bacteria, anaerobic bacteria and fungi. The aim of this study was to evaluate the effectiveness of a novel cadaver fixative solution having a 2% formaldehyde concentration. Visual examination of each fixed cadaver’s skeletal muscles and visceral organs showed that these had retained a relatively natural appearance. No yeast or mould was cultured. Anaerobic bacteria namely Clostridium sporogenes and C. tyrobutyricum were isolated from the muscles of wo sheep. The aerobic bacterium Bacillus licheniformis was cultivated from the colon and duodenum of all three horses 40 days post fixation.
Keywords
anatomy; Dissection; embalming; Formaldehyde; polyeth- ylene glycol
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