A PROTOCOL FOR MICROPROPAGATION OF Chrysanthemum morifolium, RAMAT. PLANTS | ||
| Journal of Productivity and Development | ||
| Article 5, Volume 12, Issue 2, July 2007, Pages 411-435 PDF (222.31 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/jpd.2007.44966 | ||
| Author | ||
| Azza Arafa* | ||
| Ornamental Horticulture Dept., Faculty of Agric. , Cairo Univ., Egypt. | ||
| Abstract | ||
| An in vitro propagation was carried out at Plant Tissue Culture Laboratory, Agricultural Development System Project (ADSP), Ministry of Agriculture, during the successive seasons of 2004/2005 and 2005/2006, to find out the favorable methodology for in vitro propagation of Chrysanthemum morifolium, Ramat. The results could be summarized in the following: The largest shoot length and number of leaves /shoot tip or node explants occurred due MS medium supplemented with 0.1 mg /L TDZ, whereas shoot formation (%) was the best when shoot tips or nodes were subculture in WPM medium supplied with 0.1mg /L TDZ, but callus formation favoured the 1.0 mg/l TDZ on WPM. Application GA3 at 1.0 mg /L to shoot tips, subculture on MS medium supplemented with 1.0 mg BA /L., resulted the best shoot length, number of leaves as well as shoot and callus formation. Applying 0.5 mg /L TDZ combined with 2.0 mg /L IAA to shoot tip explants gave the more number of leaves and shoot formation. While 2.0 mg /L IAA alone on shoot tips resulted the longest shoots. The heaviest callus formation occurred on node explants treated with 0.5 mg TDZ/l. Shoot tips subculture on MS medium supplemented with 1.0 mg/l BA under light intensity of 1000 lux produced the larger number of leaves and shoot length. Callus formation was the heaviest on node explants under 1000 lux light intensity. The largest number of leaves and shoot length occurred on shoot tips subculture on MS medium supplied with 1.0 mg /L BA under 240C. But,callus formation was the best on node explants under 17 0C. Adding sucrose to MS medium supplemented with 1.0 mg /L BA was the best compared to fructose and glucose as sources of carbon in producing shoot length, number of leaves and callus formation. The highest values of characters occurred due to 20 g /L sucrose combined with shoot tip explants. Shoot length and number of leaves/ shoot tip or node explants were the largest when treated with 1.0 mg /L BA, combined with 15 g. /L sucrose. Whereas number of shoots on shoot tips, or node explants was the highest at 1.0 mg /L BA with 10 g /L sucrose. The 1.0 mg /L IAA combined with 15 g /L sucrose led to the heaviest callus formation. While root length was the longest by 0.1mg /L BA combined with sucrose at 10 g /L. MS medium at 1/2 strength resulted the largest number of roots, root length and root formation, While full strength of MS failed to induce roots .Reducing MS strength caused concomitant decrease in the growth; characters shoot length and number of leaves. MS salts medium at full strength gave the largest growth characters. | ||
| Keywords | ||
| in Vitro; micro-propagation; thidiazuron (TDZ); benzyl-adenine (BA); silver nitrate (AgNO3); Chrysanthemum morifolium; Light intensity; ssucrose; type of medium-temperature | ||
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