Some Immunohistochemical Studies on the Epididymal Duct in the Donkey (Equus asinus) | ||
| Journal of Veterinary Anatomy | ||
| Article 2, Volume 2, Issue 2, October 2009, Pages 23-40 PDF (876.64 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/jva.2009.45594 | ||
| Author | ||
| Mohamed Alkafafy | ||
| Department of Cytology and Histology, Faculty of Veterinary Medicine, Minufiya University, Sadat City Branch, Egypt. | ||
| Abstract | ||
| This work aimed to study the regional distribution pattern of some biologically active proteins of the epididymal duct of donkey through application of immune-histochemistry (IHC). Paraffin-embedded sections from different regions of the epididymal duct were used. Primary antibodies against S100, angiotensinconverting enzyme (ACE), galactosyl- transferase (GalTase), vascular endo-thelial growth factor (VEGF), α-smoothmuscle actin (α-SMA) and connexin 43(Cx43) were used. The immunehistochemical findings exhibited a regional-specific, distribution pattern; different from other domestic mammals. Apical cells in the epididymal epithelium ex- pressed a moderate to strong immune-staining with all of S100, ACE and Gal-Tase. Basal cells exhibited mode-rate to strong immunoreaction with both of GalTase and VEGF. | ||
| Keywords | ||
| epididymis; donkey; IHC | ||
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