Abdel Hamid, O., Mesallam, D., Abdel-Salam, A., Zaghlol, A. (2017). GENETIC POLYMORPHISMS OF GLUTATHIONE STRANSFERASE (M1 AND T1) AND PARAOXONASE 1 (PON1) AND SUSCEPTIBILITY TO CHRONIC KIDNEY DISEASE OF UNKNOWN ETIOLOGY IN PESTICIDE EXPOSED PATIENTS AT ZAGAZIG UNIVERSITY HOSPITALS. test, 17(1), 207-222. doi: 10.21608/ejfsat.2017.46112
Omaima I. Abdel Hamid; Dalia I.A. Mesallam; Amira E Abdel-Salam; Ahmed Ahmed Mohammed Zaghlol. "GENETIC POLYMORPHISMS OF GLUTATHIONE STRANSFERASE (M1 AND T1) AND PARAOXONASE 1 (PON1) AND SUSCEPTIBILITY TO CHRONIC KIDNEY DISEASE OF UNKNOWN ETIOLOGY IN PESTICIDE EXPOSED PATIENTS AT ZAGAZIG UNIVERSITY HOSPITALS". test, 17, 1, 2017, 207-222. doi: 10.21608/ejfsat.2017.46112
Abdel Hamid, O., Mesallam, D., Abdel-Salam, A., Zaghlol, A. (2017). 'GENETIC POLYMORPHISMS OF GLUTATHIONE STRANSFERASE (M1 AND T1) AND PARAOXONASE 1 (PON1) AND SUSCEPTIBILITY TO CHRONIC KIDNEY DISEASE OF UNKNOWN ETIOLOGY IN PESTICIDE EXPOSED PATIENTS AT ZAGAZIG UNIVERSITY HOSPITALS', test, 17(1), pp. 207-222. doi: 10.21608/ejfsat.2017.46112
Abdel Hamid, O., Mesallam, D., Abdel-Salam, A., Zaghlol, A. GENETIC POLYMORPHISMS OF GLUTATHIONE STRANSFERASE (M1 AND T1) AND PARAOXONASE 1 (PON1) AND SUSCEPTIBILITY TO CHRONIC KIDNEY DISEASE OF UNKNOWN ETIOLOGY IN PESTICIDE EXPOSED PATIENTS AT ZAGAZIG UNIVERSITY HOSPITALS. test, 2017; 17(1): 207-222. doi: 10.21608/ejfsat.2017.46112

GENETIC POLYMORPHISMS OF GLUTATHIONE STRANSFERASE (M1 AND T1) AND PARAOXONASE 1 (PON1) AND SUSCEPTIBILITY TO CHRONIC KIDNEY DISEASE OF UNKNOWN ETIOLOGY IN PESTICIDE EXPOSED PATIENTS AT ZAGAZIG UNIVERSITY HOSPITALS

The Egyptian Journal of Forensic Sciences and Applied Toxicology
Article 11, Volume 17, Issue 1, June 2017, Pages 207-222    PDF (441.62 K)
Document Type: Original Article
DOI: 10.21608/ejfsat.2017.46112
Authors
Omaima I. Abdel Hamid1; Dalia I.A. Mesallam1; Amira E Abdel-Salam2; Ahmed Ahmed Mohammed Zaghlol3
1Forensic Medicine & Clinical Toxicology department, Faculty of Medicine, Zagazig University
2Community Medicine department, Faculty of Medicine, Zagazig University
3internal medicine department, Faculty of Medicine, Zagazig University
Abstract
Background: Pesticide compounds are commonly used worldwide and in Egypt. Chronic low level exposure of humans to these compounds has resulted in many health adverse effects. There are many reports relating chronic kidney disease of unknown etiology (CKDu) epidemic to pesticide exposure including organophosphates (OP). Glutathione-S-transferase mu (GSTM1) and glutathione-S-transferase theta (GSTT1) are phase II xenobiotic metabolizing enzymes. Paraxonase 1 (PON1) is an enzyme linked to high-density lipoprotein. It can prevent oxidation of low-density lipoproteins and participates in organophosphate detoxification. Objective: To investigate the association of genetic polymorphism of GST (M1, T1) and PON1 Q192R with CKDu in pesticide exposed patients presented to Nephrology Unit, Internal Medicine Department, Zagazig University Hospitals, Sharkia Governorate. Methods: Gas chromatography- mass spectrometry (GC-MS) was used for detection of dialkyl phosphate metabolites in urine samples of subjects included in the study then genomic DNA isolated from the blood samples of 48 CKDu patient chronically exposed to pesticides giving positive result for dialkyl phosphate metabolites and matched control group was used to detect the presence or absence of the GSTM1 and GSTT1 genes (gene deletion) by multiplex polymerase chain reaction technique (multiplex PCR) and PON1 polymorphisms using polymerase chain reaction–restriction fragment length polymorphism (PCR–RFLP) method. Conclusion: This study suggested that there is a correlation between chronic exposure to organophosphates and the risk of CKDu due to GSTM1 and GSTT1 genes deletions and PON1 gene polymorphisms.
Keywords
Glutathin S transferase; Paraoxonase; Chronic kidney disease of unknown etiology; Polymorphism
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