Ibrahim, I., Mansour, R., Elfayoumy, H., Ahmed, A. (2019). Oleic acid antagonizes the action of high fructose high fat diet on insulin secretion and adipose tissue β-arrestin signaling. test, 28(1), 1-12. doi: 10.21608/zjps.2019.13167.1000
Islam Ibrahim; Rehab Mansour; Hassan Elfayoumy; Ahmed Ahmed. "Oleic acid antagonizes the action of high fructose high fat diet on insulin secretion and adipose tissue β-arrestin signaling". test, 28, 1, 2019, 1-12. doi: 10.21608/zjps.2019.13167.1000
Ibrahim, I., Mansour, R., Elfayoumy, H., Ahmed, A. (2019). 'Oleic acid antagonizes the action of high fructose high fat diet on insulin secretion and adipose tissue β-arrestin signaling', test, 28(1), pp. 1-12. doi: 10.21608/zjps.2019.13167.1000
Ibrahim, I., Mansour, R., Elfayoumy, H., Ahmed, A. Oleic acid antagonizes the action of high fructose high fat diet on insulin secretion and adipose tissue β-arrestin signaling. test, 2019; 28(1): 1-12. doi: 10.21608/zjps.2019.13167.1000

Oleic acid antagonizes the action of high fructose high fat diet on insulin secretion and adipose tissue β-arrestin signaling

Zagazig Journal of Pharmaceutical Sciences
Article 1, Volume 28, Issue 1, June 2019, Pages 1-12    PDF (585.46 K)
Document Type: Original Article
DOI: 10.21608/zjps.2019.13167.1000
Authors
Islam Ibrahim* 1; Rehab Mansour2; Hassan Elfayoumy3; Ahmed Ahmed2
1Dept of pharmacology, Faculty of pharmacy
2Pharmacology and Toxicology Department, Faculty of Pharmacy, Zagazig University
3Pharmacology and Toxicology Department, Faculty of Pharmacy, Kantara Sinai University, Egypt
Abstract
Insulin resistance is a metabolic disorder associated with a wide array of cardiovascular complications. This study aimed to investigate the effect of oleic acid on a dietary model of insulin resistance in mice using high fructose high fat diet (HFrHFD) focusing on the role of β-arrestin signaling in the adipose tissue. Insulin resistance was induced by feeding mice high fructose high fat diet (HFrHFD) for 16 weeks. Oleic acid (40 mg/kg/day) was orally administered for 4 weeks starting at week 13. At the end of experiment, survival curve, body weights (BW), fasting blood glucose, serum insulin and insulin resistance (IR) were measured. Furthermore, adipose tissue levels of β-arrestin2, phosphatidyl inositol 4,5 bisphosphate (PIP2), diacyl glycerol (DAG) and phospho serine 473 of protein kinase B (pS473 Akt) were measured. Results showed that, oleic acid significantly increased survival percentage, BW and fasting blood glucose level compared to HFrHFD fed mice. On the other hand, oleic acid significantly reduced serum insulin level while slightly reduced IR compared to HFrHFD fed mice. In addition, oleic acid significantly increased β-arrestin2, PIP2 and pS473 Akt levels while significantly decreased DAG level in the adipose tissue. In conclusion, although oleic acid significantly improved survival curve and β-arrestin signaling in the adipose tissue, it worsened fasting blood glucose level in HFrHFD fed mice. An effect that may be attributed to reduced insulin secretion without comparable improvement in insulin resistance.
Keywords
Oleic acid; Insulin Resistance; β-arrestin; adipose tissue
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