Elkhateeb, S., Attia, H. (2014). Ameliorative Role of N-Acetylcysteine on Toxic Potential of Titanium Dioxide Nanoparticles on The Brain of Albino Rats. test, 22(2), 73-87. doi: 10.21608/mjfmct.2014.47659
Shereen A. Elkhateeb; Hossam F. Attia. "Ameliorative Role of N-Acetylcysteine on Toxic Potential of Titanium Dioxide Nanoparticles on The Brain of Albino Rats". test, 22, 2, 2014, 73-87. doi: 10.21608/mjfmct.2014.47659
Elkhateeb, S., Attia, H. (2014). 'Ameliorative Role of N-Acetylcysteine on Toxic Potential of Titanium Dioxide Nanoparticles on The Brain of Albino Rats', test, 22(2), pp. 73-87. doi: 10.21608/mjfmct.2014.47659
Elkhateeb, S., Attia, H. Ameliorative Role of N-Acetylcysteine on Toxic Potential of Titanium Dioxide Nanoparticles on The Brain of Albino Rats. test, 2014; 22(2): 73-87. doi: 10.21608/mjfmct.2014.47659

Ameliorative Role of N-Acetylcysteine on Toxic Potential of Titanium Dioxide Nanoparticles on The Brain of Albino Rats

Mansoura Journal of Forensic Medicine and Clinical Toxicology
Article 5, Volume 22, Issue 2, July 2014, Pages 73-87    PDF (27.71 M)
Document Type: Original Article
DOI: 10.21608/mjfmct.2014.47659
Authors
Shereen A. Elkhateeb1; Hossam F. Attia2
1Department of Forensic Medicine and Clinical Toxicology, Faculty of Medicine, Zagazig University,Egypt
2Department of Histology and Cytology, Faculty of Veterinary Medicine, Benha University
Abstract
Titanium dioxide nanoparticles (TiO2 NPs) could affect biological targets like the brain. The aim of this study was to investigate the toxic effects of TiO2 NPs on the brain of adult male rats and to evaluate the protective role of N- Acetylcysteine (NAC) on the affected parameters. Sixty adult male Albino rats were divided into 4 groups; control group, NAC group: rats received NAC (100 mg/kg), TiO2 NPs group: rats received 1200 mg/kg TiO2NPs in 1ml of 5% gum acacia solution), TiO2NPs/ NAC groups rats received TiO2 NPs and NAC as previously mentioned. The rats were gavaged once daily for 12 weeks. After 6 and 12 weeks, rats were sacrificed and their brains were obtained for estimation of brain malondialdehyde (MDA), glutathione (GSH) levels and histopathological examination. The results revealed time dependent significant increase of MDA and decreased GSH levels. Histopathological examination showed vacuoles in the brain tissues, pyknotic nuclei, necrosis in the nerve cells and fibrosis of the nerve  fibers. Bcl2 immunolocalization revealed time dependent weak reaction in the cytoplasm of nerve cells. These changes showed minimization after using NAC. The present results indicated that TiO2NPs induced time dependent oxidative stress and apoptosis in rat brain which could be ameliorated by co-administration of NAC. 
Keywords
Titanium dioxide NPs; NAC; Brain; Oxidative Stress; Bcl2; Rats
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