Isolation, identification and antimicrobial susceptibility testing of recent E. coli serotypes from Japanese Quails reared in Sharkia Governorate, Egypt | ||
| Damanhour Journal of Veterinary Sciences | ||
| Article 4, Volume 1, Issue 2, 2019, Pages 14-17 PDF (691.3 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/DJVS.38982 | ||
| Author | ||
| Walaa Fathy Ibrahim* | ||
| Educational Veterinary Hospital, Faculty of Veterinary Medicine, Zagazig University, Zagazig, Egypt | ||
| Abstract | ||
| Quails are growing sources for animal-derived protein in Egypt. This study aimed to investigate of the prevalence of avian pathogenic E. coli (APEC) in quails reared in Sharkia Governorate, Egypt. Furthermore, serological identification of APEC strains was performed followed by screening of the expression of the virulence-associated genes and the antibiogram of the identified serotypes. The achieved results in the present study revealed that E. coli isolates were recovered at 28% of the examined quail samples. E. coli isolates were recovered from the different organs at variable percentages. Serological identification of the isolated E. coli demonstrated that five serotypes were identified, namely O2, O20, O35, O78 and O127. E. coli O 127 was the most predominant serotype (28.56%). E. coli O2 came second at 24.99%. The identified serotypes harbored virulence-associated genes including tsh, astA and iroN that facilitate microbial colonization, adhesion and dissemination into different tissue. Antimicrobial resistance profiling of the identified serotypes revealed their resistance to several antimicrobials commonly used in the poultry production in Egypt including ampicillin, ceftiofur, penicillin, polymixin B and nalidixic acid. On the same time, the identified E. coli serotypes showed marked sensitivity to gentamycin, neomycin, enrofloxacin and erythromycin indicating that these antimicrobials are promising candidates for the control of E. coli infection in quails. | ||
| Keywords | ||
| E. coli; quails; Antibiogram; virulence-associated genes | ||
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