GENETICAL STUDIES OF FLAX SEED EFFECT ON LIVER AND SPLEEN OF RATS WITH NEPHROPATHY. | ||
| Journal of Agricultural Chemistry and Biotechnology | ||
| Article 6, Volume 6, Issue 12, December 2015, Pages 675-686 PDF (593.72 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/jacb.2015.48476 | ||
| Authors | ||
| Amina M. G. Zedan1; M. Rehan2; Nabila Y. Mahmoud3 | ||
| 1Biological and Environmental Science Department, Faculty of Home Economics, Al Azhar University, Tanta, Gharbia Governorate, Egypt. | ||
| 2Genetic Department, Faculty of Agriculture, Kafrelsheikh University, Kafr El-Sheikh Governorate, Egypt. | ||
| 3Nutrition and Food Sciences Department, Faculty of Home Economics, Al Azhar University, Tanta, Gharbia Governorate, Egypt. | ||
| Abstract | ||
| The flax seed is rich with lignans and α-linolenic acid plus antioxidant effects. The purpose of this study was to estimate the effect of flax seed on recovery of acute renal failure in rats. Twenty four rats were divided into four groups (each group has six individuals). One group was used as negative control (n=6), whereas the other three groups (n=18) injected with glycerol to induce acute renal failure. One of three groups fed on basal diet as positive control (C+), while the second and third groups (FS1 and FS2) was fed on 5 and 7% flax seeds for 28 days. In acute renal failure by glycerol in rats, kidneys, liver and heart weight was significantly increased when compared with negative control. RAPD amplifies the target genomic DNA with five primers generated a total of 116, 142 DNA fragments in liver and spleen, respectively. In liver, fifteen band of thirty eight (39.47 %) were polymorphic, whereas twenty three bands were monomorphic for all treatments. The highest level of polymorphism (55.56%) was observed in primer 5 and the lowest level of polymorphism was 16.7% in primer 1. In spleen, total of bands resulted from five random primer was forty two bands, the polymorphic bands were sixteen (38.1 %) and the monomorphic band were twenty six bands (61.9 %). | ||
| Keywords | ||
| Flax seed; acute renal failure; DNA polymorphic | ||
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