Impact of Antioxidant Supplementation to Maturation Medium on In Vitro Maturation of Rabbit Oocytes Collected from Cryopreserved Ovaries | ||
| Journal of Animal and Poultry Production | ||
| Article 2, Volume 7, Issue 6, June 2016, Pages 203-208 PDF (247.74 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/jappmu.2016.48702 | ||
| Authors | ||
| A. Z. Mehrez1; Sh. M. Shamiah2; Rania R. Ali1 | ||
| 1Animal Prod. Dept., Faculty of Agric., Mansoura Univ. | ||
| 2Anim. Prod. Res. Inst., Ministry of Agric. | ||
| Abstract | ||
| The aim of the present study was to evaluate the effect of adding two types of antioxidants, namely vitamin E (VE) or glutathione reduced (GSH) on in vitro maturation of New Zealand white (NZW) rabbit oocytes recovered from ovaries exposed to vitrification solution or vitrification as compared to fresh ovaries. A total of 36 non-parous NZW rabbit does, ranging 4.5-5 months of age and 2.25-2.55 kg LBW, were used in this study. Oocytes were recovered from ovaries exposed to vitrification solution, vitrified ovaries and fresh ovaries. Oocytes were in vitro matured in TCM-199 medium supplemented with 0, 200 µM VE or 200 µM GSH in CO2 incubator (5% CO2) at 37.5°C and high humidity for 18 h. Results show that maturation rate in term of percentage of oocytes reached MII stage was the highest (P<0.05) for oocytes recovered from fresh ovaries, moderate for those recovered from exposed ovaries and the lowest for oocytes recovered from vitrified ovaries (60.19, 37.16 and 29.05%, respectively). This trend was associated with an opposite trend in percentage of degenerated oocytes (7.88, 13.40 and 18.09%, respectively). Regardless cryopreservation method of rabbit ovary, the effect of both types of antioxidant (VE and GSH) supplementation on the proportion of maturation rate of rabbit oocytes is revealed that he percentage of oocytes reached MII stage was higher (P<0.01) for oocytes matured by antioxidant than by control medium. The percentage of matured oocytes was higher (50.66%) for oocytes matured by GSH than VE (43.96%) versus the lowest percentage of control oocytes at MII stage (31.77%). However, oocytes at other stages was significantly (P<0.05) decreased for oocytes matured by GSH as compared to control, but those matured by VE did not significantly differ from that in GSH and control oocytes. Analysis of variance revealed that the effect of interaction between oocyte preservation and antioxidant supplementation was not significant on percentage of oocyte at different stages after IVM. Maturation rate of fresh, exposed or vitrified oocytes increased when they were in vitro matured in medium supplemented with antioxidant as compared to un-supplemented medium, being higher with GSH than VE. In conclusion, based on the foregoing results, oocytes recovered from ovaries exposed to vitrification solution or vitrified showed lower maturation rate than those recovered from fresh ovaries. | ||
| Keywords | ||
| Rabbit; Ovary; Vitrification; in vitro maturation; antioxidant | ||
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