Ellakany, H., Elbestawy, A., Sayed-Ahmed, A., Elgammal, S., Gado, A., Abdel-Hameed, H. (2019). Genetic point mutation inducing antigenic drift in hypervariable region of a very virulent IBDV isolate in chickens in Egypt during 2014-2016. test, 2(1), 12-17. doi: 10.21608/djvs.2019.49553
Hany Ellakany; Ahmed Elbestawy; Ahmed Sayed-Ahmed; Samia Elgammal; Ahmed Gado; Hatem Abdel-Hameed. "Genetic point mutation inducing antigenic drift in hypervariable region of a very virulent IBDV isolate in chickens in Egypt during 2014-2016". test, 2, 1, 2019, 12-17. doi: 10.21608/djvs.2019.49553
Ellakany, H., Elbestawy, A., Sayed-Ahmed, A., Elgammal, S., Gado, A., Abdel-Hameed, H. (2019). 'Genetic point mutation inducing antigenic drift in hypervariable region of a very virulent IBDV isolate in chickens in Egypt during 2014-2016', test, 2(1), pp. 12-17. doi: 10.21608/djvs.2019.49553
Ellakany, H., Elbestawy, A., Sayed-Ahmed, A., Elgammal, S., Gado, A., Abdel-Hameed, H. Genetic point mutation inducing antigenic drift in hypervariable region of a very virulent IBDV isolate in chickens in Egypt during 2014-2016. test, 2019; 2(1): 12-17. doi: 10.21608/djvs.2019.49553

Genetic point mutation inducing antigenic drift in hypervariable region of a very virulent IBDV isolate in chickens in Egypt during 2014-2016

Damanhour Journal of Veterinary Sciences
Article 3, Volume 2, Issue 1, 2019, Pages 12-17    PDF (832.88 K)
Document Type: Original Article
DOI: 10.21608/djvs.2019.49553
Authors
Hany Ellakany* 1; Ahmed Elbestawy1; Ahmed Sayed-Ahmed2; Samia Elgammal1; Ahmed Gado1; Hatem Abdel-Hameed1
1Department of Poultry and Fish Diseases, , Faculty of Veterinary Medicine, Damanhour University
2Department of Anatomy and Embryology, Faculty of Veterinary Medicine, Menoufia University, Shebin ElKoum-Menofia 32511, Egypt
Abstract
            Infectious bursal disease (IBD) is a highly contagious viral disease affecting young chickens causing immune suppression, high morbidity and mortality. Its economic significance is recognized worldwide. In this study, suspected IBD samples (bursa of Fabricious) were collected from 45 chicken flocks in 3 Egyptian governorates from 2014 to 2016. The virus was inoculated in embryonated chicken eggs via chorio-allantoic- membrane (CAM) route inducing specific IBDV lesions in the embryos. Viral identification was carried out through Reverse Transcriptase Polymerase Chain Reaction (RT-PCR) targeting VP2 gene. Fourteen positive IBDV isolates (31%) were confirmed by RT-PCR. Three pure IBDV isolates were subjected to partial VP2 gene sequence analysis from which 2 IBDV isolates No. 1 and 3 (Accession No. KX827589.1 and MK906027) were defined as a very virulent IBDV (vvIBDV) genogroup 3, while the third isolate (Accession No. KX827588.1) was closely related to a vaccine strain in cvIBDV genogroup 1. Nucleotide and amino acid sequence analysis and blast of the IBDV isolates indicated a close relationship with the previously recorded Egyptian IBDVs with 96 to 99% identity. Point mutation or amino acid substitution in positions P202M (conserved region); and A211T, D212Y (hypervariable region) of the VP2 gene in the isolate No. 3 vvIBDV (Accession No. MK906027) that differ from all the previously recorded Egyptian isolates in GenBank were present.
Keywords
IBDV; RT-PCR; Antigenic mutation; chickens
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