Histological and immunohistochemical study on the potential toxicity of sliver nanoparticles on the structure of the spleen in adult male albino rats | ||
| Egyptian Journal of Histology | ||
| Article 9, Volume 40, Issue 3, September 2017, Pages 374-387 PDF (8.69 M) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/EJH.2017.4662 | ||
| Authors | ||
| Nehad F. Mazen* 1; Ebtehal Z. Saleh2; Abeer A. Mahmoud2; Aly A. Shaalan3 | ||
| 1Department of Histology and Cell Biology, Faculty of Medicine, Zagazig University1 | ||
| 2Department of Histology and Cell Biology, Faculty of Medicine, Zagazig University | ||
| 3Department of Histology and Cell Biology, Faculty of Medicine, Suez Canal University | ||
| Abstract | ||
| Introduction: Silver nanoparticles (AgNPs) remain one of the controversial research areas regarding their distribution, dissolution and their toxicity to biological systems. Aim of the work: To evaluate the potential toxicity of different doses of AgNPs on the spleen of adult Albino rats. Materials and Methods: Forty adult male albino rats were divided into two main groups; control group: included ten rats that received intraperitoneal saline solution, daily, and experimental group: included thirty rats that received intraperitoneal AgNPs dissolved in saline solution, and were equally subdivided into three subgroups. Subgroup IIa received AgNPs (100 mg/kg/d), subgroup IIb received AgNPs (500 mg/kg/d) and subgroup IIc received AgNPs (1000 mg/kg/d). After 28 days, rats were euthanized and the spleen specimens were prepared for light and electron microscope studies. Results: Spleen of AgNPs-treated rats revealed dose-dependent structural changes in the form of reduced sized white pulp follicles with depletion of lymphocytes, degeneration and apoptosis. Nanoparticles were seen in both white and red pulps. There was a highly statistical significant increase in the optical density of caspase-3 in subgroups IIa, IIb and IIc when compared to that of the control group. Conclusions: AgNPs induced structural alterations in the spleen with evidence of inflammatory response and oxidative stress. These effects were dose-dependent. | ||
| Keywords | ||
| rat; silver nanoparticles; spleen; Ultrastructure | ||
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