Cost Effective Culture Medium for Micropropagation of Paulownia (Paulownia tomentosa Steud.) and Catalpa (Catalpa bignonioides Walt.) | ||
| المجلة العربية للعلوم الزراعية | ||
| Article 2, Volume 2, Issue 4, October 2019, Pages 33-46 PDF (760.06 K) | ||
| Document Type: المقالة الأصلية | ||
| DOI: 10.21608/asajs.2019.52886 | ||
| Author | ||
| Rafail S. Toma | ||
| Department of Horticulture, College of Agriculture, University of Duhok Iraqi Kurdistan Region | ||
| Abstract | ||
| أجريت محاولة للحصول على وسط غذائي متوازن ذو تکاليف اقتصادية قلبلة للاکثار الدقيق لنباتي الباولونيا والکتالبا من خلال اختبار عدة أوساط غذائية وهي MS وWPM وGD مع اضافة تراکيز متباينة من BA وNAA في المراحل الاکثار المتوالية. أظهرت النتائج على الحصول على 100% من المزارع المعقمة من خلال تعقيم البذور لکلا النباتين في 2.5% من هايبوکلورات الصوديوم لمدة 10 دقائق. وصلت نسب انبات البذور خارج الجسم الحي للباولونيا الى 98% وللکتالبا الى 95% على التوالي. عند زراعة العقد المتبادلة کأجزاء نباتية في وسط MS المزود بـ2.0 ملغم/ لتر من BA تم الحصول على أکبر عدد من الفروع للجزء النباتي (2.46 فرع/ جزء نباتي). بينما أقل عدد من الفروع (1.37 فرع/ جزء نباتي) تم الحصول عليه من خلال الزراعة في وسط GD الخالي من BA. أطول الفروع تم الحصول عليها من خلال الزراعة في وسط WPM المزود بـ 2.0 ملغم/ لتر BA (1.39 سم). اضافة BA قد أدى الى زيادة عدد الفروع ومتوسط الطول للکتالبا (1.86 فرع/ جزء نباتي). أکبر عدد من الفروع (1.92) تم الحصول عليه عند الزراعة في وسط MS المزود بـ 2.00 ملغم/ لتر BA. إضافة 1.0 ملغمم لتر من NAA ادت الى زيادة عدد الجذور الى 6.84 جذرواعلى نسبة تجذير وصلت الى 100% للباولونيا النامية في وسط WPM. أعلى نسبة تجذير (100%) وأطول الجذور (4.88 سم) تم تسجيلها من خلال الزراعة في وسط WPM الخالي من NAA. اضافة 1.0 ملغم/ لتر من NAA الى WPM کان کفيلاً قي زيادة عدد الجذور الى 14.26 جذر/ جزء نباتي ونسبة التجذير الى 98% للکتالبا. تم أقلمة ونقل النبيتات الناتجة بنجاح الى خارج المختبر بنسبة نجاح وصلت الى 100% لکلا النباتين. Abstract: An attempt was done to achieve a cost effective culture medium for paulownia and catalpa micropropagation. Different types of culture media including MS, WPM and GD were tested with the addition of BA and NAA at various concentration in the successive micropropagation stages. The results showed that 100% aseptic cultures were obtained through disinfesting the seeds of both plants in 2.5% sodium hypochlorite for 10 minutes. In vitro seed germination rates of paulownia and catalpa reached to 98% and 95% respectively. Paulownia alternative node explants grown on MS medium enriched with 2.0 mg.l-1 BA gave the highest number of shoots per explant (2.46 shoots/ explant). Whereas, the least number of shoots (1.37 shoots/ explant) were recorded for explants grown on GD medium with 00.0 mg.l-1 BA. The longest shoots were found for explants grown on WPM medium enriched with 2.0 mg.l-1 BA (1.39 cm). The addition of BA was increased the number of shoots per explants and the mean length of shoots of catalpa by giving 1.86 shoots/ explant and 1.29 cm respectively. The highest number of shoots per explant 1.92 shoots/ explant was recorded for the explants grown on MS medium with 2.0 mg/l-1 BA. The addition of 1.0 mg.l-1 NAA raised the number of roots per explant by producing 6.84 roots/ explant and the highest rooting percentage (100%) on paulownia micro-shoots grown on WPM. The highest rooting percentage (100%) and longest roots (4.88 cm) were found on micro-shoots grown on NAA-free WPM. The addition of 1.0 mg.l-1 NAA to WPM was significantly effective in raising the number of roots per explant to reach 14.26 roots/ explant and the rooting percentage to 98% for catalpa micro-shoots. NAA-free WPM produced the longest roots (4.40 cm) with the 98% of rooting. A 100% successful acclimatization process was achieved for both tree plantlets. | ||
| Keywords | ||
| in Vitro; MS; WPM; GD; growth regulators | ||
| References | ||
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