MORPHOLOGICAL CHARACTERIZATION AND In vitro DEVELOPMENT OF VITRIFIED EMBRYOS FROM BALADI AND APRI RABBIT | ||
| Journal of Animal and Poultry Production | ||
| Article 3, Volume 6, Issue 8, August 2015, Pages 555-566 PDF (236.02 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/jappmu.2015.52915 | ||
| Authors | ||
| H. N. Fahim* ; M. A Shitifa; Samia Z. Meshreky | ||
| Animal Production Research Institutes, Agriculture Research center, Dokki, Giza, Egypt, | ||
| Abstract | ||
| A total of 185 morphologicaly normal embryos were collected from 32 superovulated multiparous baladi black (BB) and APRI donor rabbits. Embryos collected were divided into three groups. The first group (n=63) was non-vitrified (used as fresh control) group and the second group (n=60) was vitrified by one step ((DMSO alone), the third group (n=62) was vitrified by two step (DMSO+EG). Embryos vitrified by one and two step methods storage in liquid nitrogen at 15 day post thawing. Results showed that the two-steps vitrification procedure gave insignificantly better results of post-thawing morphologically normal embryos grade excellent (38.71%) compared to the one-step vitrification procedure (31.67%). The total morphological normal embryo recovered post- thawing in excellent and good grades as affected by vitrification procedure and genotype (breeds) showed that BB was high insignificantly compared with APRI vitrified by the two steps vitrification method (77.42 vs. 74.19%) or one step (72.41 vs. 70.97%). The fresh APRI embryos gave significantly (P<0.05) total embryo diameter thick compared with the same embryo breed vitrified by one or two step methods (424.8±3.8 vs 365.8±3.6 and 360.5±3.5 µm). The BB rabbit have the smallest total embryo diameter vitrified by two or one step methods (351.7±4.0 and 357.1±4.0µm) compared to BB intact embryo (409.4±4.1 µm). Only 19/60 (31.67%) vitrified embryos by one step method reached hatching blastocyst stages in culture medium post thawing compared to 28/62 (45.16%) vitrified embryo by two steps, the difference was significant. The highest percentages of embryos cleaved in vitro reached hatching blastocysts stage have been obtained from APRI (75.00%) and BB (74.19%) un-vitrified embryos, followed by BB (48.39%) and APRI (41.93%) embryos vitrified-thawing by two steps vitrification method, whereas the lower percentages were recorded with embryos vitrified-thawing by one step procedure in APRI (32.26%) and BB (31.03%) embryos. The present study demonstrate that the two step vitrification protocol (DMSO+EG) provides superior embryo survival rates over the one step (DMSO alone) vitrification protocol for morulae stage rabbit embryos for Baladi black or ABRI rabbits. | ||
| Keywords | ||
| Vitrification; one step; two step; rabbit breed | ||
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