Biodegradation of diesel oil wastes for production of biosurfactants by two Actinomycetes strains | ||
| Research Journal of Applied Biotechnology | ||
| Article 6, Volume 1, Issue 1, June 2015, Pages 64-79 PDF (606.61 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/rjab.2015.53532 | ||
| Authors | ||
| Ayman Eldourghamy* 1; Sabha Elsabagh* 2; Abdallah Ismail* 2 | ||
| 1Environmental Biotechnology Department, GEBRI, University of Sadat City | ||
| 2Department of Microbiology, Faculty of Science, University of Minufiya | ||
| Abstract | ||
| Biotechnological studies on production of bio-surfactants by actinomycetes strains that were isolated from Egyptian soil were carried out using some diesel oil waste samples. Two actinomycetes strains were found to give bio-surfactant production up to 40 - % when tested with emulsification test. Effect of carbon and nitrogen sources on bio-surfactant production were tested using urea, ammonium nitrate, ammonium chloride and potassium nitrate. Best results for bio-surfactant production were observed with urea and ammonium nitrate as the main nitrogen source in the biological medium. Best results for bio-surfactant production were observed when using Bushnell Hass medium supplement with % hydrocarbon waste. Ammonium sulfate precipitated samples were further applied on Sephadex LH-20 column to obtain purified products. Results obtained from isolated strains show optimistic results for a successful bioremediation of diesel oil wastes for production of commercial active bio-surfactants that could be used in both industrial and pharmaceutical fields. | ||
| Keywords | ||
| Bio-surfactants; biodegradation; Actinomycetes; LH-20 column | ||
| References | ||
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