Expression of Hypoxia Inducible Factor-1a Correlates with Microvessel Density in Glioblastoma Multiforme | ||
| The Medical Journal of Cairo University | ||
| Article 40, Volume 87, June, June 2019, Pages 1675-1679 PDF (337.88 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/mjcu.2019.53924 | ||
| Author | ||
| MOHAMED WAGIH, M.D. | ||
| The Department of Pathology, Faculty of Medicine, Beni-Suef University, Egypt | ||
| Abstract | ||
| Abstract Background: Glioblastoma multiforme (GBM) is the most aggressive brain tumour characterized by marked angiogenesis, invasiveness and poor outcome. Intratumoral hypoxia possibly plays a key role in GBM growth and angiogenesis. A key regulator of adaptive response to hypoxia is the hypoxia inducible factor 1-alpha (HIF-1a) protein. Aim of Study: The present study aimed to evaluate the expression of HIF-1a in tumour cells of GBM and analyze its relation to tumour microvessel density. Material and Methods: Fifty formalin fixed paraffin embedded tissue blocks of GBM were studied immunohisto-chemically for HIF-1a expression using anti-HIF-1a mono-clonal antibody and for microvessel density using anti-CD34 monoclonal antibody. Results: HIF-1a expression was observed in 86% of the studied cases. The immuostaining scores were negative in 14%, weak positive in 20%, moderate positive in 28% and strong positive in 38% of cases The mean microvessel counts were 14.3±11.5 in the negative cases, 21.6±15.8 in the weak positive cases, 27.2±21.7 in the moderate positive cases and 32.8±16.3 in the strong positive cases. A statistically significant relation was found between the expression of HIF-1a protein and microvessel density in the studied GBM cases (p=0.01). Conclusion: Our findings have shown that expression of HIF-1a protein is correlated to the microvessel density in GBM, supporting evidence that different types of tumours can induce aberrant angiogenesis through HIF-1a. Thus, an understanding of the relationship between HIF-1a and tumour angiogenesis in GBM may provide further therapeutic oppor-tunities for patients with this tumour. | ||
| Keywords | ||
| Glioblastoma Multiforme; Hypoxia Inducible Factor-1a; Microvessel Density | ||
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