abdallah, O., EL-Baghdady, K., Khalil, M., El Borhamy, M., Meligi, G. (2019). Biosynthesis, Characterization and Cytotoxicity of Selenium Nanoparticles Using Bacillus tropicus Ism 2. test, 11(1), 47-57. doi: 10.21608/eajbsg.2019.55269
omnia mohamed abdallah; Khaled Z. EL-Baghdady; Mostafa M.H. Khalil; Mervat I. El Borhamy; Gamal Meligi. "Biosynthesis, Characterization and Cytotoxicity of Selenium Nanoparticles Using Bacillus tropicus Ism 2". test, 11, 1, 2019, 47-57. doi: 10.21608/eajbsg.2019.55269
abdallah, O., EL-Baghdady, K., Khalil, M., El Borhamy, M., Meligi, G. (2019). 'Biosynthesis, Characterization and Cytotoxicity of Selenium Nanoparticles Using Bacillus tropicus Ism 2', test, 11(1), pp. 47-57. doi: 10.21608/eajbsg.2019.55269
abdallah, O., EL-Baghdady, K., Khalil, M., El Borhamy, M., Meligi, G. Biosynthesis, Characterization and Cytotoxicity of Selenium Nanoparticles Using Bacillus tropicus Ism 2. test, 2019; 11(1): 47-57. doi: 10.21608/eajbsg.2019.55269

Biosynthesis, Characterization and Cytotoxicity of Selenium Nanoparticles Using Bacillus tropicus Ism 2

Egyptian Academic Journal of Biological Sciences, G. Microbiology
Article 6, Volume 11, Issue 1, December 2019, Pages 47-57    PDF (874.74 K)
Document Type: Original Article
DOI: 10.21608/eajbsg.2019.55269
Authors
omnia mohamed abdallah1; Khaled Z. EL-Baghdady2; Mostafa M.H. Khalil3; Mervat I. El Borhamy4; Gamal Meligi3
1Department of Microbiology, Faculty of Dentistry, Misr International University, Cairo, Egypt
2Department of Microbiology, Faculty of Science, Ain Shams University, Cairo, Egypt
3Department of Chemistry, Faculty of Science, Ain Shams University, Cairo, Egypt
4-Department of Microbiology, Faculty of Pharmacy, Misr International University, Cairo, Egypt -International Medical Center, Clinical Microbiology Laboratory, Cairo, Egypt
Abstract
    Green biosynthesis of selenium nanoparticles (SeNPs) using Bacillus tropicus Ism 2 (MK332444) isolated from soil polluted with industrial wastewater near the Ismailia Canal, Egypt, was carried out. SeNPs were biogenically synthesized using bacterial pellets mixed with 1 mM Na2SeO3. Characterization of biosynthesized SeNPs was carried out by UV-Vis spectrum where SeNPs solution exhibited characteristic peak at 270 nm. Dynamic Light Scattering (DLS) analysis and High-Resolution Transmission Electron Microscope (HRTEM) showed spherical shaped SeNPs with size ranged from 60 to 125 nm with PDI= 0.544 and zeta potential value of -30.6. Biologically synthesized SeNPs showed no antibacterial activity against both Gram-positive and Gram-negative bacteria. Cytotoxicity of SeNPs was studied against Huh-7 human liver cell line and showed low cytotoxicity with IC50 value of367±5.5 μg/ml. In conclusion in this work Bacillus tropicus Ism 2 locally isolated bacterium showed the capability to biosynthesize SeNPs with spherical shape and low cytotoxicity. 
Keywords
Selenium nanoparticles; DLS; TEM; Cytotoxicity; Bacillus tropicus Ism 2
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