Ahmed, A., Hussein, A., Abd Elmagid, A. (2019). Molecular Characterization of Shiga Toxin Producing Escherichia coli Isolated From Both Diarrheic and Apparently Health Calves. test, 1(2), 1-10. doi: 10.21608/jcvr.2019.56993
Amira Ahmed; Alaa Eldin Hussein; Abd Elrahman Abd Elmagid. "Molecular Characterization of Shiga Toxin Producing Escherichia coli Isolated From Both Diarrheic and Apparently Health Calves". test, 1, 2, 2019, 1-10. doi: 10.21608/jcvr.2019.56993
Ahmed, A., Hussein, A., Abd Elmagid, A. (2019). 'Molecular Characterization of Shiga Toxin Producing Escherichia coli Isolated From Both Diarrheic and Apparently Health Calves', test, 1(2), pp. 1-10. doi: 10.21608/jcvr.2019.56993
Ahmed, A., Hussein, A., Abd Elmagid, A. Molecular Characterization of Shiga Toxin Producing Escherichia coli Isolated From Both Diarrheic and Apparently Health Calves. test, 2019; 1(2): 1-10. doi: 10.21608/jcvr.2019.56993

Molecular Characterization of Shiga Toxin Producing Escherichia coli Isolated From Both Diarrheic and Apparently Health Calves

Journal of Current Veterinary Research
Article 1, Volume 1, Issue 2, November 2019, Pages 1-10    PDF (546.49 K)
Document Type: Original Article
DOI: 10.21608/jcvr.2019.56993
Authors
Amira Ahmed* 1; Alaa Eldin Hussein2; Abd Elrahman Abd Elmagid3
1Department of Bacteriology, Animal Health Research Institute, Alexandria Provincial Lab., Dokky, Giza, Egypt
2Department of Bacteriology ,Mycology and Immunity, Faculty of Veterinary Medicine, University of Sadat City, Egypt.
3Department of Bacteriology, Animal Health Research Institute, Alexandria Provincial Lab., Dokky, Giza, Egypt.
Abstract
Shiga toxin producing Escherichia coli is a contaminant of food and water that causes a diarrheal syndrome followed by more severe disease of the kidneys and symptoms of the central nervous system in humans. The isolation of Shiga toxin producing Escherichia coli (STEC) from diarrheic and apparently health calves is difficult due to lack of differential phenotypic characteristics from nonpathogenic Escherichia coli. The improvement of molecular technology allows identification of both toxin and serogroup specific genetic determinants. In this study, 300 fecal samples from diarrheic and apparently healthy calves were screened for STEC using PCR targeting Shiga toxin determinants. In addition routine culture methods for isolating O157 and non O157 STEC were also performed. The screening assays of serotyping isolates revealed 7 (4.1%) of O157H7, 156 (92.8%) of non O157 and 5 (3.1%) for untypable strains. These included STEC serotypes of O157H7 and O26 from diarrheic samples, and O78, O55 and O126 from apparently healthy calves. The high rate of STEC isolation and the diversity of STEC serogroup from calves focus the light on the importance of calves as reservoir of E. coli as well as motivate us to improve biosecurity measures in dairy farms.
Keywords
Escherichia coli; Shiga toxin; Serotyping; O157 H7; Non O157; diarrheic and apparently health calves
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