Characterization of Salmonella spp. Isolated From Poultry Giblets, Calves and Human Beings in Menoufiya Governorate | ||
| Journal of Current Veterinary Research | ||
| Article 9, Volume 1, Issue 2, November 2019, Pages 78-94 PDF (543.15 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/jcvr.2019.57059 | ||
| Authors | ||
| Sara Elwaraqi* ; Ahmed Bayomi; Sherif Zidan | ||
| Department of Animal Hygiene and Zoonoses , Faculty of Veterinary Medicine , University of Sadat City , Egypt. | ||
| Abstract | ||
| Salmonellosis remains as an important public health problem and of economic importance worldwide. The aim of this study was to indicate the epidemiology of salmonellosis in both animal and human being in Menofiya Governorate. A total of 352 samples from poultry giblets (52 liver, 32 gizzard, 30 heart), frozen minced meat (58), calves faeces (50) and 130 stool samples were collected and examined bacteriologically, biochemically. Our results revealed that the prevalence of Salmonella spp. was 71.15% (37/52), 59.4% (19/32), 60% (18/30), 24.14% (14/58), 6% (3/50) and 42.3% (55/130) in liver, gizzard, heart, frozen minced meat, calves fecal matter and human stool, respectively. Moreover, 10 isolates of them had been serotyped and for further confirmation 33 isolates from these sources were examined using PCR to detect presence of Salmonella species invA gene and virulence genes (mgtC and hilA). By serotyping S.typhimurium was detected in liver, heart, frozen minced meat and calves fecal matter, S.enteritidis was found in gizzard and human stool samples, while S.kentucky was only detected from human stool samples. Furthermore, PCR on 33 isolates detected (100%) for invA, (84.85%) for mgtC and (90.9%) for hilA gene. In conclusion, Salmonella species has potential zoonotic spread and the presence of virulence genes in isolates from animals, products and in-contact human being, confirming the role of food from animal origin in transmission of salmonellosis. | ||
| Keywords | ||
| Salmonella spp; XLD; Serotyping; invA; hilA; mgtC | ||
|
Statistics Article View: 412 PDF Download: 473 |
||
