Salman, N., El-Tekeya, M., Bakry, N., Soliman, S. (2019). REMINERALIZATION EFFECT OF FLUORIDE VARNISH CONTAINING CASEIN PHOSPHOPEPTIDE AMORPHOUS CALCIUM PHOSPHATE ON CARIES-LIKE LESIONS IN PRIMARY TEETH (IN VITRO STUDY). test, 44(1), 13-16. doi: 10.21608/adjalexu.2019.57568
Nehal R. Salman; Magda M. El-Tekeya; Niveen S. Bakry; Samia Soliman. "REMINERALIZATION EFFECT OF FLUORIDE VARNISH CONTAINING CASEIN PHOSPHOPEPTIDE AMORPHOUS CALCIUM PHOSPHATE ON CARIES-LIKE LESIONS IN PRIMARY TEETH (IN VITRO STUDY)". test, 44, 1, 2019, 13-16. doi: 10.21608/adjalexu.2019.57568
Salman, N., El-Tekeya, M., Bakry, N., Soliman, S. (2019). 'REMINERALIZATION EFFECT OF FLUORIDE VARNISH CONTAINING CASEIN PHOSPHOPEPTIDE AMORPHOUS CALCIUM PHOSPHATE ON CARIES-LIKE LESIONS IN PRIMARY TEETH (IN VITRO STUDY)', test, 44(1), pp. 13-16. doi: 10.21608/adjalexu.2019.57568
Salman, N., El-Tekeya, M., Bakry, N., Soliman, S. REMINERALIZATION EFFECT OF FLUORIDE VARNISH CONTAINING CASEIN PHOSPHOPEPTIDE AMORPHOUS CALCIUM PHOSPHATE ON CARIES-LIKE LESIONS IN PRIMARY TEETH (IN VITRO STUDY). test, 2019; 44(1): 13-16. doi: 10.21608/adjalexu.2019.57568

REMINERALIZATION EFFECT OF FLUORIDE VARNISH CONTAINING CASEIN PHOSPHOPEPTIDE AMORPHOUS CALCIUM PHOSPHATE ON CARIES-LIKE LESIONS IN PRIMARY TEETH (IN VITRO STUDY)

Alexandria Dental Journal
Article 3, Volume 44, Issue 1, April 2019, Pages 13-16    PDF (96.5 K)
DOI: 10.21608/adjalexu.2019.57568
Authors
Nehal R. Salman* 1; Magda M. El-Tekeya2; Niveen S. Bakry3; Samia Soliman4
1Instructor, Department of Pediatric Dentistry, Faculty of Dentistry, Pharos University, Egypt
2Professor of Pediatric Dentistry, Department of Pediatric Dentistry and Dental Public Health, Faculty of Dentistry, Alexandria University, Egypt
3Professor of Pediatric Dentistry, Faculty of Dentistry, Alexandria University, Alexandria, Egypt.
4Professor of Oral Biology, Department of Oral Biology, Faculty of Dentistry, Alexandria University, Egypt.
Abstract
INTRODUCTION: Fluoride containing casein phosphopeptide amorphous calcium phosphate (CPP-ACP) varnishes are now available as (MI Varnish). It has an effect on the remineralization process of demineralized enamel, since it has the highest release of calcium and fluoride ions. OBJECTIVES: To study the remineralizing effect of fluoride varnish containing casein phosphopeptide amorphous calcium phosphate (MI varnish) on the remineralization of caries-like lesions in primary teeth compared to sodium fluoride varnish. MATERIALS AND METHODS: Twenty-four freshly extracted primary maxillary incisors with standardized 4x4 windows were immersed in demineralizing solution for four days. Teeth were divided into two groups, group I (n=12 teeth); MI varnish and group II (n=12 teeth); Prevident varnish. They were sectioned into two halves in a buccolingual direction (48 specimens). One half was treated with the remineralizing agent and the other half remained untreated and served as its control. Specimens were assigned into subgroup I A (MI varnish) n=12 specimens, subgroup I B (untreated) n=12 specimens, subgroup II A (Prevident varnish) n=12 specimens and subgroup II B (untreated) n=12 specimens. Specimens were subjected to pH cycling for ten days. The specimens were evaluated quantitatively using energy dispersive x-ray spectrometer (EDX). RESULTS: MI varnish treated specimens (subgroup I A) showed significant high mean Ca, Ca/P ratio than the untreated specimens. Also, Prevident varnish treated specimens showed significant high mean Ca, Ca/P ratio than the untreated specimens. Median percent change of Ca/P ratio of MI varnish treated specimens was significantly higher than Prevident varnish treated specimens. CONCLUSIONS: Fluoride varnish containing CPP-ACP has a higher remineralization potential of the caries-like lesion in primary teeth in comparison to sodium fluoride varnish.
Keywords
(MI varnish); casein phosphopeptide amorphous calcium phosphate (CPP-ACP); remineralization; fluoride varnish; primary teeth; energy dispersive x-ray spectrometer
References
  1. Cury JA, Tenuta LM. Enamel remineralization controlling the caries disease or treating early carious lesions. Braz Oral Res. 2009; 23: 23-30.
  2. Cochrane NJ, Cai F, Huq NL, Burrow MF, Reynolds EC. New Approaches to enhanced remineralization of tooth enamel. J Dent Res. 2010; 89: 1187-97.
  3. Schemhron BR, Wood GD. Comparison of fluoride uptake into tooth enamel from two fluoride varnishes containing different calcium fluoride sources. J Clin Dent. 2011; 22; 51-4.
  4. Marinho VCC, Worthington HV, Walsh T, Clarkson JE. Fluoride varnishes for preventing dental caries in children and adolescents. Cochrane Database Syst Rev. 2013; 7; 8-18.
  5. Reynolds EC. Calcium phosphate-based remineralization systems: scientific evidence. Aust Dent J. 2008; 53: 268- 73.
  6. Cochrane NJ, Shen P, Yuan Y, Reynolds EC. Ion release from calcium and fluoride containing dental varnishes. Aust Dent J. 2014; 59: 100-5.
  7. Seppa L. Fluoride varnishes in caries prevention. Med Princ Pract. 2004;13: 307-11.
  8. Pradeep K, Prasanna RK. Remineralizing agents in the non-invasive treatment of early carious lesions. Int J Dent Case Reports. 2011; 1: 73-84.
  9. Llena C, Forner L, Baca P. Anticariogenicity of casein phophopeptide-amorphous calcium phosphate: a review of the literature. J Contemp Dent Pract. 2009; 10: 1-9.
  10. Gupta R, Prakash V. CPP-ACP complex as a new adjunctive agent for remineralisation: a review. Oral Health Prev Dent. 2011; 9:151-65.
  11. Heikal M. The effect of casein phosphopeptide amorphous calcium phosphate on remineralization of caries-like lesions in primary teeth. Master thesis. Faculty of Dentistry, Alexandria University; 2012.
  12. GC Company America. Instructions for use of MI varnish. Available at: http://www.gcamerica.com/products/preventive/MI_Varni sh/. Accessed on March 2015.
  13. Shen P, Bagheri R, Walker GD, Yuan Y, Stanton DP, Reynolds C, et al. Effect of calcium phosphate addition to fluoride containing dental varnishes on enamel demineralization. Aust Dent J. 2016; 61: 357-65.
  14. Rirattanapong P, Vongsavan K, Saengsirinavin C, Pornmahala T. Effect of fluoride varnishes containing tricalcium phosphate sources remineralization of initial primary enamel lesions. Southeast Asian J Trop Med Public Health. 2014; 45: 499-502.
  15. Yoshaskam A, Namratha LP, Gaurav P, Thajuraj PK. The effect of CCP-ACP on remineralization of artificial caries lesions: an in vitro study. Indian J Multidiscip Dent. 2011; 2: 366-77.
  16. ten Cate JM, Duijesters PPE. Alternating demineralization and remineralization of artificial enamel lesions. Caries Res. 1982; 16: 201-10.
  17. Kumar VLN, Itthagarun A, King NM. The effect of casein phosphopeptide-amorphous calcium phosphate on remineralization of artificial caries-like lesions: an in vitro study. Aust Dent J. 2008; 53: 34-40.
  18. Colgate® company Prevident® Varnish. Instructions for use for prevident varnish. Available at: http://www.colgateprofessional.com/products/colgateprevident-varnish-rx-only/information. Accessed on March 2015.
  19. Cardoso CA, de Castilho AR, Salomão PM, Costa EN, Magalhães AC, Buzalaf MA. Effect of xylitol varnishes on remineralization of artificial enamel caries lesions in vitro. J Dent. 2014; 42: 1495-501.
  20. Itthagarun A, Wei SH, Wefel JS. The effect of different commercial dentifrices on enamel lesion progression: an in vitro pH-cycling study. Int Dent J. 2000; 50: 21-8.
  21. De Carvalho Filho AC, Sanches RP, Martin AA, Do Espírito Santo AM, Soares LE. Energy Dispersive XRay Spectrometry Study of the Protective Effects of Fluoride Varnish and Gel on Enamel Erosion. Microsc Res Tech. 2011; 74: 839-44.
  22. Savas S, Kavrik F, Kucukyilmaz E. Evaluation of the remineralization capacity of CPP-ACP containing fluoride varnish by different quantitative methods. J Appl Oral Sci. 2016; 24: 198-203.
  23. Hicks J, Garcia-Godoy F, Flaitz C. Biological factors in dental caries: role of remineralization and fluoride in the dynamic process of demineralization and remineralization (part 3). J Clin Pediatr Dent. 2004; 28: 203-14.
  24. Oliveira M, Mansur HS. Synthetic tooth enamel: SEM characterization of a fluoride hydroxyapatite coating for dentistry application. Master Res. 2007; 10: 115-8.
  25. Kariya S, Sato T, Sakaguchi Y, Yoshii E. Fluoride effect on acid resistance capacity of CPP-ACP containing material. Abstract 2045. 82nd General Session of the IADR 2004, Honolulu, Hawaii. Available at: http://www.the-ozone.cc/research/abstracts/ab147.pdf. Accessed on November 2016.
  26. Lippert F, Hara AT, Martinez-Mier EA, Zero DT. Laboratory investigations into the potential anticaries efficacy of fluoride varnishes. Pediatr Dent. 2014; 36: 291-5.
Statistics
Article View: 774
PDF Download: 1,564