DETECTING THE ANTI-STREPTOCOCCUS PYOGENS ACTIVITY OF CYANOBACTERIUM (APHANOCAPSA SPECIES) BY USING PLACKETT-BURMAN EXPERIMENTAL DESIGN | ||
| Research Journal of Applied Biotechnology | ||
| Article 12, Volume 3, Issue 1, June 2017, Pages 36-46 PDF (537.46 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/rjab.2017.57642 | ||
| Authors | ||
| Amira M. El Farakh1; Ragaa A. Hamouda1; Mohamed O. Abdel Fatah2; Saad I. Amar3 | ||
| 1Microbial biotechnology department, Genetic Engineering and Biotechnology research Institute, University Of Sadat City, Sadat city, Egypt | ||
| 2Molecular Biology, Genetic Engineering and Biotechnology research Institute, University Of Sadat City, Sadat city, Egypt | ||
| 3Faculty of Medicine, Banha University, Alqalyubia, Egypt. | ||
| Abstract | ||
| Streptococcus pyogenes is associated with a wide range of infections and disease states, that infects children and adolescents causing sore throat. This study was carried out to evaluate the potential of exopolysaccharides of Aphanocapsa sp was tested for antibacterial activity against Streptococcus pyogenes ( Group A streptococcus) bacterial cultures using well diffusion method.Plackett- Burman design was applied on original BG11medium to determine the best conditions that effect on activity of exopolysaccharides of Aphanocapsasp against Streptococcus pyogenes. Plackett- Burman design was performed on original BG11medium by applied various nitrogen sources NaNO3and ammonium chloride. It was found that exopolysacchaaride extracted from Aphanocapsa sp had activity against Streptococcus pyogenes. Exopolysacchaaride extracted from alga that grown in Bg11medium supplemented with ammonium chloride had more effective against Streptococcus pyogenes than that obtained from Bg11medium supplemented with NaNO3. | ||
| Keywords | ||
| Antibacterial; Aphanocapsa sp; Streptococcus pyogenes; Cyanobacteria; Plackett- Burman design | ||
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