Ibrahim, I., Karam, S., Aly, H. (2018). BIOMIMETIC ENAMEL REMINERALIZATION USING CHITOSAN HYDROGEL (AN IN VITRO STUDY). test, 43(3), 116-121. doi: 10.21608/adjalexu.2018.58011
Ingy E. Ibrahim; Sahar S. Karam; Hanaa M. Aly. "BIOMIMETIC ENAMEL REMINERALIZATION USING CHITOSAN HYDROGEL (AN IN VITRO STUDY)". test, 43, 3, 2018, 116-121. doi: 10.21608/adjalexu.2018.58011
Ibrahim, I., Karam, S., Aly, H. (2018). 'BIOMIMETIC ENAMEL REMINERALIZATION USING CHITOSAN HYDROGEL (AN IN VITRO STUDY)', test, 43(3), pp. 116-121. doi: 10.21608/adjalexu.2018.58011
Ibrahim, I., Karam, S., Aly, H. BIOMIMETIC ENAMEL REMINERALIZATION USING CHITOSAN HYDROGEL (AN IN VITRO STUDY). test, 2018; 43(3): 116-121. doi: 10.21608/adjalexu.2018.58011

BIOMIMETIC ENAMEL REMINERALIZATION USING CHITOSAN HYDROGEL (AN IN VITRO STUDY)

Alexandria Dental Journal
Article 19, Volume 43, Issue 3, December 2018, Pages 116-121    PDF (805.65 K)
Document Type: Original Article
DOI: 10.21608/adjalexu.2018.58011
Authors
Ingy E. Ibrahim1; Sahar S. Karam2; Hanaa M. Aly3
1DBS – Faculty of Dentistry - Alexandria University.
2Professor and Head of Oral Biology Department - Faculty of Dentistry - Alexandria University, Egypt.
3Professor of Oral Biology – Faculty of Dentistry – Alexandria University
Abstract
INTRODUCTION: Biomimetic enamel reconstruction is a significant topic in material science and dentistry as a novel approach for the treatment of dental caries or erosion. Compared to other conventional treatments, biocompatible and biodegradable chitosan hydrogel, with its antimicrobial properties shows promise as a biomaterial for the prevention, restoration, and treatment of defective enamel. OBJECTIVES: The present study was designed to investigate the effect of chitosan on the remineralization of enamel. MATERIALS AND METHODS Sound human premolars extracted for orthodontic reasons and were sectioned into 1mm thick slices.30 slices were selected and randomly divided into 3 equal groups (10 slices per group) as follows: GroupI: Control group, the slices were etched with 37% phosphoric acid for 30 seconds. Group II: The slices were etched with 37% phosphoric acid for 30 seconds, coated with bonding agent and chitosan hydrogel. Then slices were incubated at 37ºC in artificial saliva solution for 5 days. Group III: The slices were etched with 37% phosphoric acid for 30 seconds, coated with bonding agent and chitosan hydrogel. Then slices were incubated at 37ºC in artificial saliva solution for 10 days. After remineralization, the enamel surfaces were ultrasonically cleaned for 2 minutes and analyzed with scanning electron microscope (SEM) in order to observe induced structural changes on the enamel surface then elemental analysis and Ca/P ratios were carried out using Energy Dispersive Analysis X-Ray (EDAX) spectrometer. The obtained data were statistically analyzed. RESULTS: Morphological changes on the enamel surface after application of chitosan observed by SEM revealed the regeneration of an enamel-like layer in both treated samples. There was a statistically significant difference between the Ca/P ratios of the treatment groups compared to that of the control group. CONCLUSIONS: The ultrastructural and elemental alterations noticed in this study substantiate the use of chitosan in cases of dental erosions
Keywords
enamel; remineralization; Chitosan
References
  1. Cao Y, Mei ML, Li QL, Lo EC, Chu CH. Enamel prismlike tissue regeneration using enamel matrix derivative. J Dent. 2014;42:1535-42.
  2. Dawes C. What is the critical pH and why does a tooth dissolve in acid? J Can Dent Assoc. 2003;69:722-4.
  3. Sudjalim TR, Woods MG, Manton DJ. Prevention of white spot lesions in orthodontic practice: a contemporary review. Aust Dent J. 2006;51:284-9.
  4. Nobre-dos-Santos M, Rodrigues LK, Del-Bel-Cury AA, Cury JA. In situ effect of a dentifrice with low fluoride concentration and low pH on enamel remineralization and fluoride uptake. J Oral Sci. 2007;49:147-54.
  5. Newby CS, Creeth JE, Rees GD, Schemehorn BR. Surface microhardness changes, enamel fluoride uptake, and fluoride availability from commercial toothpastes. J Clin Dent. 2006;17:94-9.
  6. Young A, Thrane PS, Saxegaard E, Jonski G, Rölla G. Effect of stannous fluoride toothpaste on erosion-like lesions: an in vivo study. Eur J Oral Sci. 2006;114:180-3.
  7. Magalhães AC, Stancari FH, Rios D, Buzalaf MA. Effect of an experimental 4% titanium tetrafluoride varnish on dental erosion by a soft drink. J Dent. 2007;35:858-61.
  8. Skrtic D, Hailer AW, Takagi S, Antonucci JM, Eanes ED. Quantitative assessment of the efficacy of amorphous calcium phosphate/methacrylate composites in remineralizing caries-like lesions artificially produced in bovine enamel. J Dent Res. 1996;75:1679-86.
  9. Reynolds EC, Cai F, Shen P, Walker GD. Retention in plaque and remineralization of enamel lesions by various forms of calcium in a mouth rinse or sugar-free chewing gum. J Dent Res. 2003;82:206-11.
  10. Skrtic D, Eanes ED, Antonucci JM. Polymeric calcium phosphate composites with remineralization potential. Industrial Biotechnological Polymers 1995; 393-408.
  11. Hannig M, Hannig C. Nanotechnology and its role in caries therapy. Adv Dent Res. 2012;24:53-7.
  12. Li X, Pan D, Lin S, Zhuang Z, Linb Z. Facile in vitro hydroxyapatite remineralization of human enamel with remarkable hardness. Cryst Eng Comm. 2013;15:4351.
  13. Bianco A, Cacciotti I, Lombardi M, Montanaro L, Gusmano G. Thermal Stability And Sintering Behaviour Of Hydroxyapatite Nanopowders. JTAC. 2007;88:237-43.
  14. Wilcock CJ, Gentile P, Hatton PV, Miller CA. Rapid Mix Preparation of Bioinspired Nanoscale Hydroxyapatite for Biomedical Applications. J Vis Exp. 2017;(120).
  15. Chen L, Yuan H, Tang B, Liang K, Li J. Biomimetic remineralization of human enamel in the presence of polyamidoamine dendrimers in vitro. Caries Res. 2015;49: 282-90.
  16. Han M, Li QL, Cao Y, Fang H, Xia R, Zhang ZH. In vivo remineralization of dentin using an agarose hydrogel biomimetic mineralization system. Sci Rep. 2017;7: 41955.
  17. Zikakis JP. Chitosan and Related Enzymes. Chitin, Orlando, Fla: Academic Press; 1984. p XVII.
  18. Jay S, Dutta PK. Preparation, antibacterial and physicochemical behavior of chitosan/ofloxacin complexes. Int J Polym Mater. 2010;59:793-807.
  19. Oyane A, Kawashita M, Nakanishi K, Kokubo T, Minoda M, Miyamoto T, et al. Bonelike apatite formation on ethylene-vinyl alcohol copolymer modified with silane coupling agent and calcium silicate solutions. Biomaterials. 2003;24:1729-35.
  20. Yamaguchi I, Itoh S, Suzuki M, Osaka A, Tanaka J. The chitosan prepared from crab tendons: II. The chitosan/apatite composites and their application to nerve regeneration. Biomaterials. 2003;24:3285-92.
  21. de Carvalho MMSG, Stamford TCM, dos Santos EP, Tenório P, Sampaio F. Chitosan as an oral antimicrobial agent. Science against microbial pathogens: communicating current research and technological advances A. Méndez-Vilas (Ed.) 2011. Available at : http://www.formatex.org/polymerscience1/chapters/542- 550.pdf
  22. Ruan Q, Moradian-Oldak J. Development of Amelogeninchitosan Hydrogel for In Vitro Enamel Regrowth with a Dense Interface. J Vis Exp. 2014;51606.
  23. Xu Z, Neoh KG, Lin CC, Kishen A. Biomimetic deposition of calcium phosphate minerals on the surface of partially demineralized dentine modified with phosphorylated chitosan. J Biomed Mater Res B Appl Biomater. 2011;98:150-9.
  24. Tian K, Pengb M, Fei W, Ren XH. Induced Synthesis of Hydroxyapatite by Chitosan for Enamel Remineralization. Adv Mat Res. 2012:530:40-5.
  25. Wang Y, Li B, Zhou Y, Jia D. In Situ Mineralization of Magnetite Nanoparticles in Chitosan Hydrogel. Nanoscale Res Lett. 2009;4:1041-6.
  26. Han M, Li QL, Cao Y, Fang H, Xia R, Zhang ZH. In vivo remineralization of dentin using an agarose hydrogel biomimetic mineralization system. Sci Rep. 2017;7:41955.
  27. Li B, Wang Y, Jia D, Zhou Y. Gradient structural bone-like apatite induced by chitosan hydrogel via ion assembly. J Biomater Sci Polym Ed. 2011;22:505-17.
  28. Husain S, Al-Samadani KH, Najeeb S, Zafar MS, Khurshid Z, Zohaib S, et al. Chitosan Biomaterials for Current and Potential Dental Applications. Materials (Basel). 2017;10. pii: E602.
  29. Das UM, G S. Bonding agents in pit and fissure sealants: a review. Int J Clin Pediatr Dent. 2009;2:1-6.
  30. Cao Y, Mei ML, Li QL, Lo EC, Chu CH. Agarose hydrogel biomimetic mineralization model for the regeneration of enamel prismlike tissue. ACS Appl Mater Interfaces. 2014;6:410-20.
  31. Wu X, Zhao X, Li Y, Yang T, Yan X, Wang K. In situ synthesis carbonated hydroxyapatite layers on enamel slices with acidic amino acids by a novel two-step method. Mater Sci Eng C Mater Biol Appl 2015;54:150-7.
  32. Cao CY, Mei ML, Li QL, Lo EC, Chu CH. Methods for biomimetic remineralization of human dentine: A systematic review. Int J Mol Sci. 2015;16:4615-27.
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