Method Development and Validation of Carbendazim Fungicide in Cucumber تطوير واعتماد طريقة لتحليل المبيد الفطرى الکاربندازيم فى الخيار | ||
| Journal of Plant Protection and Pathology | ||
| Article 2, Volume 10, Issue 8, August 2019, Pages 397-400 PDF (658.33 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/jppp.2019.58178 | ||
| Authors | ||
| علاء مسعود خزيمى1; محمد فتحی عبد الرحمن رمضان2 | ||
| 1قسم وقاية النبات - کلية الزراعة - جامعة دمنھور - مصر | ||
| 2مرکزالبحوث الزراعية - المعمل المرکزي للمبيدات - قسم بحوث تحليل المبيدات - الدقي - الجيزة – مصر | ||
| Abstract | ||
| Evaluation of pesticide residues was carried out in cucumber to determine the residues of carbendazim which collected from different local markets in Egypt. During a 14-day, treated cucumbers samples were gathered and analyzed to determine the carbendazim residue quantity. A QuEChERS sample preparation has been applied with liquid chromatography provided with mass spectrometry (LC/MS-MS) to evaluate residual of tested fungicide. Two transitions ions was obtained from multiple reaction monitoring (MRM) after that higher sensitivity transition used for quatification but the lower sensitivity transitions used to confirmation analysis of carbendazin. The method was validated by setting performance parameters such as linearity, precision, recovery, limits of detection (LOD) (0.45 µg Kg1) and limits of quantification (LOQ) (0.9 µg Kg1). Good correlation coefficient R2 0,9992 in pure solvent curve and R2 1.000 for matrix-matched curve, which given the elevated calibration curve quality. The carbendazin recoveries founded in fresh cucumber samples of 10 replicates were found to be in the range between 119.11 to 124.45% (% RSD > 3.5) for two fortification levels (0.010 and 0.070 mgKg1). The intra-day repeatability RSD value was 3.26%, while the inter-day repeatability RSD value was 5.27%. Matrix effect of carbendazim in cucumber was evaluated and the results showed that carbendazim had a soft matrix effect (6.53).Samples of tested fungicides showed distinct contamination levels up to a certain time period (7 days), after which no residues were identified between 11-14 days. | ||
| Keywords | ||
| Pesticide residue; Ultra Performance Liquid Chromatography; MS Detector; Carbendazim | ||
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